Overview

  • Product name
    Anti-ZEB1 antibody [2A8A6]
    See all ZEB1 primary antibodies
  • Description
    Mouse monoclonal [2A8A6] to ZEB1
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ZEB1 aa 967-1108. Expressed in E. coli.
    Sequence:

    KRFS HSGSYSQHMN HRYSYCKREA EERDSTEQEE AGPEILSNEH VGARASPSQG DSDERESLTR EEDEDSEKEE EEEDKEMEEL QEEKECEKPQ GDEEEEEEEE EVEEEEVEEA ENEGEEAKTE GLMKDDRAES QASSLGQK


    Database link: P37275

  • Positive control
    • WB: Human ZEB1 recombinant protein and HAP1 cell lysate. IHC-P: Human cervical cancer and rectum cancer tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR224480-10 are from Tissue Culture Supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    2A8A6
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 124 kDa.

Target

  • Function
    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
  • Tissue specificity
    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
  • Involvement in disease
    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
  • Sequence similarities
    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AREB 6 antibody
    • AREB6 antibody
    • BZP antibody
    • Delta crystallin enhancer binding factor 1 antibody
    • DELTA EF1 antibody
    • FECD6 antibody
    • MGC133261 antibody
    • Negative regulator of IL 2 antibody
    • Negative regulator of IL2 antibody
    • NIL 2 A antibody
    • NIL 2 A zinc finger protein antibody
    • NIL 2A antibody
    • NIL-2-A zinc finger protein antibody
    • NIL2A antibody
    • Posterior polymorphous corneal dystrophy 3 antibody
    • PPCD3 antibody
    • Represses interleukin 2 expression antibody
    • TCF 8 antibody
    • TCF-8 antibody
    • TCF8 antibody
    • Transcription factor 8 (represses interleukin 2 expression) antibody
    • Transcription factor 8 antibody
    • ZEB 1 antibody
    • ZEB antibody
    • ZEB1 antibody
    • ZEB1_HUMAN antibody
    • ZFHEP antibody
    • ZFHX 1A antibody
    • ZFHX1A antibody
    • Zinc finger E box binding homeobox 1 antibody
    • Zinc finger E-box-binding homeobox 1 antibody
    • Zinc finger homeodomain enhancer binding protein antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ZEB1 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab181451 observed at 200 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab181451 was shown to recognize ZEB1 in wild-type HAP1 cells as signal was lost at the expected MW in ZEB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ZEB1 knockout samples were subjected to SDS-PAGE. Ab181451 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : KEB1 (AA: 967-1108)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 124 kDa

  • Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution + ZEB1 recombinant protein fragment (Expected MW is 41.7 kDa)

    Predicted band size: 124 kDa

  • Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.

  • Immunofluorescence analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow cytometric analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Negative control (red).

References

This product has been referenced in:
  • Qiao B  et al. MicroRNA-27a-3p Modulates the Wnt/ß-Catenin Signaling Pathway to Promote Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Stem Cells by Targeting SFRP1. Sci Rep 7:44688 (2017). WB ; Human . Read more (PubMed: 28425477) »
  • Deng Y  et al. MiR-429 suppresses the progression and metastasis of osteosarcoma by targeting ZEB1. EXCLI J 16:618-627 (2017). Read more (PubMed: 28694763) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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