Anti-ZEB1 antibody (ab124512)
Key features and details
- Rabbit polyclonal to ZEB1
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ZEB1 antibody
See all ZEB1 primary antibodies -
Description
Rabbit polyclonal to ZEB1 -
Host species
Rabbit -
Specificity
ab124512 is predicted not to crossreact with ZEB2. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ZEB1 (internal sequence).
Database link: P37275 -
Positive control
- WB: HeLa whole cell lysate (ab150035). ICC: HeLa and K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
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| ICC |
Use a concentration of 20 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa. |
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ICC
Use a concentration of 20 µg/ml. |
Target
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Function
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
Tissue specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
Involvement in disease
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
Sequence similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6935 Human
- Omim: 189909 Human
- SwissProt: P37275 Human
- Unigene: 124503 Human
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Alternative names
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
Images
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Western blot - Anti-ZEB1 antibody (ab124512)Anti-ZEB1 antibody (ab124512) at 1 µg/ml + HeLa cell lysate at 15 µg
Predicted band size: 124 kDa -
Immunocytochemistry - Anti-ZEB1 antibody (ab124512)Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20µg/ml.
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Immunocytochemistry - Anti-ZEB1 antibody (ab124512)Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (33)
ab124512 has been referenced in 33 publications.
- Zhao L et al. lncRNA SNHG17 promotes pancreatic carcinoma progression via cross-talking with miR-942. Am J Transl Res 13:1037-1050 (2021). PubMed: 33841638
- Dong D et al. Silencing of long non-coding RNA PCAT6 restrains gastric cancer cell proliferation and epithelial-mesenchymal transition by targeting microRNA-15a. Gen Physiol Biophys 39:1-12 (2020). PubMed: 32039820
- Fan P et al. Promotion effects of mono-2-ethyhexyl phthalate (MEHP) on migration and invasion of human melanoma cells via activation of TGF-ß signals. Cell Biochem Funct 38:38-46 (2020). PubMed: 31667872
- Xiang W et al. The lncRNA SNHG5-mediated miR-205-5p downregulation contributes to the progression of clear cell renal cell carcinoma by targeting ZEB1. Cancer Med 9:4251-4264 (2020). PubMed: 32281285
- Wang M et al. circ_KIAA1429 accelerates hepatocellular carcinoma advancement through the mechanism of m6A-YTHDF3-Zeb1. Life Sci 257:118082 (2020). PubMed: 32653519
