Anti-ZEB1 antibody (ab124512)
Key features and details
- Rabbit polyclonal to ZEB1
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZEB1 antibody
See all ZEB1 primary antibodies -
Description
Rabbit polyclonal to ZEB1 -
Host species
Rabbit -
Specificity
ab124512 is predicted not to crossreact with ZEB2. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ZEB1 (internal sequence).
Database link: P37275 -
Positive control
- WB: HeLa whole cell lysate (ab150035). ICC: HeLa and K562 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa. | |
| ICC | Use a concentration of 20 µg/ml. |
Target
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Function
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
Tissue specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
Involvement in disease
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
Sequence similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6935 Human
- Omim: 189909 Human
- SwissProt: P37275 Human
- Unigene: 124503 Human
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Alternative names
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
Images
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Western blot - Anti-ZEB1 antibody (ab124512)Anti-ZEB1 antibody (ab124512) at 1 µg/ml + HeLa cell lysate at 15 µg
Predicted band size: 124 kDa -
Immunocytochemistry - Anti-ZEB1 antibody (ab124512)Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20µg/ml.
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Immunocytochemistry - Anti-ZEB1 antibody (ab124512)Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.
Protocols
Datasheets and documents
References (23)
ab124512 has been referenced in 23 publications.
- Du X et al. LINC00511 contributes to glioblastoma tumorigenesis and epithelial-mesenchymal transition via LINC00511/miR-524-5p/YB1/ZEB1 positive feedback loop. J Cell Mol Med 24:1474-1487 (2020). PubMed: 31856394
- Zou X et al. Long Noncoding RNA LINC00460 Modulates MMP-9 to Promote Cell Proliferation, Invasion and Apoptosis by Targeting miR-539 in Papillary Thyroid Cancer. Cancer Manag Res 12:199-207 (2020). PubMed: 32021436
- Yang Z et al. Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy. Onco Targets Ther 13:989-1000 (2020). PubMed: 32099402
- Dai B et al. WD Repeat Domain 5 Promotes Invasion, Metastasis and Tumor Growth in Glioma Through Up-Regulated Zinc Finger E-Box Binding Homeobox 1 Expression. Cancer Manag Res 12:3223-3235 (2020). PubMed: 32440219
- Zhou F et al. Knockdown of ubiquitin-specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc-finger E-box binding homeobox 1. Mol Med Rep 22:1382-1390 (2020). PubMed: 32468048
