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    zeb1-antibody-ab124512.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZEB1 antibody (ab124512)

  • Datasheet
Submit a review Q&A (1)References (23)

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Western blot - Anti-ZEB1 antibody (ab124512)
  • Immunocytochemistry - Anti-ZEB1 antibody (ab124512)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)
  • Immunocytochemistry - Anti-ZEB1 antibody (ab124512)

Key features and details

  • Rabbit polyclonal to ZEB1
  • Suitable for: ICC/IF, WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human ZEB1 protein (ab152728)
Primary
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ZEB1 antibody
    See all ZEB1 primary antibodies
  • Description

    Rabbit polyclonal to ZEB1
  • Host species

    Rabbit
  • Specificity

    ab124512 is predicted not to crossreact with ZEB2.
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ZEB1 (internal sequence).
    Database link: P37275

  • Positive control

    • WB: HeLa whole cell lysate (ab150035). ICC: HeLa and K562 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cancer
    • Signal transduction
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ZEB1 protein (ab152728)

Applications

Our Abpromise guarantee covers the use of ab124512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
ICC Use a concentration of 20 µg/ml.

Target

  • Function

    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
  • Tissue specificity

    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
  • Involvement in disease

    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
  • Sequence similarities

    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P37275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6935 Human
    • Omim: 189909 Human
    • SwissProt: P37275 Human
    • Unigene: 124503 Human
    • Alternative names

      • AREB 6 antibody
      • AREB6 antibody
      • BZP antibody
      • Delta crystallin enhancer binding factor 1 antibody
      • DELTA EF1 antibody
      • FECD6 antibody
      • MGC133261 antibody
      • Negative regulator of IL 2 antibody
      • Negative regulator of IL2 antibody
      • NIL 2 A antibody
      • NIL 2 A zinc finger protein antibody
      • NIL 2A antibody
      • NIL-2-A zinc finger protein antibody
      • NIL2A antibody
      • Posterior polymorphous corneal dystrophy 3 antibody
      • PPCD3 antibody
      • Represses interleukin 2 expression antibody
      • TCF 8 antibody
      • TCF-8 antibody
      • TCF8 antibody
      • Transcription factor 8 (represses interleukin 2 expression) antibody
      • Transcription factor 8 antibody
      • ZEB 1 antibody
      • ZEB antibody
      • ZEB1 antibody
      • ZEB1_HUMAN antibody
      • ZFHEP antibody
      • ZFHX 1A antibody
      • ZFHX1A antibody
      • Zinc finger E box binding homeobox 1 antibody
      • Zinc finger E-box-binding homeobox 1 antibody
      • Zinc finger homeodomain enhancer binding protein antibody
      see all

    Images

    • Western blot - Anti-ZEB1 antibody (ab124512)
      Western blot - Anti-ZEB1 antibody (ab124512)
      Anti-ZEB1 antibody (ab124512) at 1 µg/ml + HeLa cell lysate at 15 µg

      Predicted band size: 124 kDa

    • Immunocytochemistry - Anti-ZEB1 antibody (ab124512)
      Immunocytochemistry - Anti-ZEB1 antibody (ab124512)

      Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)
      Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)

      Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20µg/ml.

    • Immunocytochemistry - Anti-ZEB1 antibody (ab124512)
      Immunocytochemistry - Anti-ZEB1 antibody (ab124512)

      Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (23)

    Publishing research using ab124512? Please let us know so that we can cite the reference in this datasheet.

    ab124512 has been referenced in 23 publications.

    • Du X  et al. LINC00511 contributes to glioblastoma tumorigenesis and epithelial-mesenchymal transition via LINC00511/miR-524-5p/YB1/ZEB1 positive feedback loop. J Cell Mol Med 24:1474-1487 (2020). PubMed: 31856394
    • Zou X  et al. Long Noncoding RNA LINC00460 Modulates MMP-9 to Promote Cell Proliferation, Invasion and Apoptosis by Targeting miR-539 in Papillary Thyroid Cancer. Cancer Manag Res 12:199-207 (2020). PubMed: 32021436
    • Yang Z  et al. Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy. Onco Targets Ther 13:989-1000 (2020). PubMed: 32099402
    • Dai B  et al. WD Repeat Domain 5 Promotes Invasion, Metastasis and Tumor Growth in Glioma Through Up-Regulated Zinc Finger E-Box Binding Homeobox 1 Expression. Cancer Manag Res 12:3223-3235 (2020). PubMed: 32440219
    • Zhou F  et al. Knockdown of ubiquitin-specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc-finger E-box binding homeobox 1. Mol Med Rep 22:1382-1390 (2020). PubMed: 32468048
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Bonjour,

    j'ai commandé récemment chez vous la référence suivante : ab64098 (Anti-AREB6) ; cependant vous venez de m'informer que vous n'en avez plus en stock et que le délai de réapprovisionnement est indéterminé. Vous m'avez donc proposé des anticorps que vous jugez comme une solution alternative dont cette référence : ab124512, cependant cet anticorps réagit chez l'humain alors que nous travaillons chez la souris.
    Pouvez-vous nous envoyer un échantillon gratuit afin de le tester ?

    Je vous remercie d'avance

    Cordialement

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2012

    Answer

    Merci pour votre émail et toutes mes excuses pour le délais de ma réponse.

    Malheureusement nous n'avons pas des petits échantillons de nos produits.Pour savoir si ca vaux la peine de tester le ab124512 pour une crossréactivité contre la souris, j'ai contacté le laboratoire afin de savoir si nous pouvonsprédire la crossréactivitébasé sur un homologie de la séquence. Malheureusement il y a seulement environs62% de homologie avec l'immunogène et la séquence de souris. Il est donc très peu probable que cet anticorps va crossréagir avec des échantillons de souris.

    Autrement nous avons aussi le ab81972 qui est un anticorps contrel le AREB6, testé en WB et sur la souris. Cet anticorps est doncgarantie par nous pour cette application.Si l'anticorps ne fonctionne pas comme décrit sur la fiche technique, nousallons le remplacer.Click here (or use the following: https://www.abcam.com/ZEB1-antibody-ab81972.html).

    J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres question.

    Read More

    Abcam Scientific Support

    Answered on Jul 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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