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    zeb1-antibody-ab155249.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZEB1 antibody (ab155249)

  • Datasheet
  • SDS
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ChIP - Anti-ZEB1 antibody (ab155249)
  • Western blot - Anti-ZEB1 antibody (ab155249)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab155249)
  • Immunoprecipitation - Anti-ZEB1 antibody (ab155249)

Key features and details

  • Rabbit polyclonal to ZEB1
  • Suitable for: ChIP, IP, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ZEB1 antibody
    See all ZEB1 primary antibodies
  • Description

    Rabbit polyclonal to ZEB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ZEB1 aa 254-586 (internal sequence).
    Database link: P37275

  • Positive control

    • ChIP: HeLa cell chromatin extract. WB: Neuro-2a, C8-D30, NIH/3T3, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: HeLa cells. IP: HEK-293T cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cancer
    • Signal transduction
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Anti-ZEB1 antibody (ab124512)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Anti-ZEB1 antibody (ab87280)
  • Recombinant Protein

    • Recombinant Human ZEB1 protein (ab152728)
  • Related Products

    • Anti-ZEB1 antibody (ab81972)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP
Use at an assay dependent concentration.
IP
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
ICC/IF
1/100 - 1/1000.
Notes
ChIP
Use at an assay dependent concentration.
IP
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
  • Tissue specificity

    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
  • Involvement in disease

    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
  • Sequence similarities

    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P37275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6935 Human
    • Entrez Gene: 21417 Mouse
    • Omim: 189909 Human
    • SwissProt: P37275 Human
    • SwissProt: Q64318 Mouse
    • Unigene: 124503 Human
    • Unigene: 3929 Mouse
    • Alternative names

      • AREB 6 antibody
      • AREB6 antibody
      • BZP antibody
      • Delta crystallin enhancer binding factor 1 antibody
      • DELTA EF1 antibody
      • FECD6 antibody
      • MGC133261 antibody
      • Negative regulator of IL 2 antibody
      • Negative regulator of IL2 antibody
      • NIL 2 A antibody
      • NIL 2 A zinc finger protein antibody
      • NIL 2A antibody
      • NIL-2-A zinc finger protein antibody
      • NIL2A antibody
      • Posterior polymorphous corneal dystrophy 3 antibody
      • PPCD3 antibody
      • Represses interleukin 2 expression antibody
      • TCF 8 antibody
      • TCF-8 antibody
      • TCF8 antibody
      • Transcription factor 8 (represses interleukin 2 expression) antibody
      • Transcription factor 8 antibody
      • ZEB 1 antibody
      • ZEB antibody
      • ZEB1 antibody
      • ZEB1_HUMAN antibody
      • ZFHEP antibody
      • ZFHX 1A antibody
      • ZFHX1A antibody
      • Zinc finger E box binding homeobox 1 antibody
      • Zinc finger E-box-binding homeobox 1 antibody
      • Zinc finger homeodomain enhancer binding protein antibody
      see all

    Images

    • ChIP - Anti-ZEB1 antibody (ab155249)
      ChIP - Anti-ZEB1 antibody (ab155249)

      Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab155249. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

      ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9.

    • Western blot - Anti-ZEB1 antibody (ab155249)
      Western blot - Anti-ZEB1 antibody (ab155249)
      All lanes : Anti-ZEB1 antibody (ab155249) at 1/1000 dilution

      Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
      Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
      Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 124 kDa



      5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab155249)
      Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab155249)

      Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ZEB1 at nucleus (green) with ab155249 at 1/500 dilution. Red: Phalloidin, a cytoskeleton marker, at 1/100 dilution.

      Scale bar = 10 μm.

    • Immunoprecipitation - Anti-ZEB1 antibody (ab155249)
      Immunoprecipitation - Anti-ZEB1 antibody (ab155249)

      IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

      A - 40 μg of HEK-293T whole cell lysate.

      B - Control with 2 μg of preimmune rabbit IgG.

      C - Immunoprecipitation of ZEB1 protein by 2 μg of ab155249.

      The immunoprecipitated ZEB1 protein was detected by ab155249 diluted at 1:1000, followed by anti-rabbit IgG.

      7.5% SDS-PAGE

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab155249? Please let us know so that we can cite the reference in this datasheet.

    ab155249 has been referenced in 11 publications.

    • Nappi A  et al. The NANOG Transcription Factor Induces Type 2 Deiodinase Expression and Regulates the Intracellular Activation of Thyroid Hormone in Keratinocyte Carcinomas. Cancers (Basel) 12:N/A (2020). PubMed: 32197405
    • Liu J  et al. Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFß/SMAD2 axis in vitro. BMC Mol Cell Biol 21:65 (2020). PubMed: 32928102
    • Wu T  et al. Mir-30b-5p Promotes Proliferation, Migration, and Invasion of Breast Cancer Cells via Targeting ASPP2. Biomed Res Int 2020:7907269 (2020). PubMed: 32420372
    • Jiang M  et al. Downregulation of PFTK1 Inhibits Migration and Invasion of Non-Small Cell Lung Cancer. Onco Targets Ther 13:9281-9289 (2020). PubMed: 33061417
    • Chen YY  et al. Protein arginine methyltranferase-1 induces ER stress and epithelial-mesenchymal transition in renal tubular epithelial cells and contributes to diabetic nephropathy. Biochim Biophys Acta Mol Basis Dis 1865:2563-2575 (2019). PubMed: 31199999
    View all Publications for this product

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