Anti-ZEB1 antibody (ab155249)
Key features and details
- Rabbit polyclonal to ZEB1
- Suitable for: ChIP, IP, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZEB1 antibody
See all ZEB1 primary antibodies -
Description
Rabbit polyclonal to ZEB1 -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ZEB1 aa 254-586 (internal sequence).
Database link: P37275 -
Positive control
- ChIP: HeLa cell chromatin extract. WB: Neuro-2a, C8-D30, NIH/3T3, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: HeLa cells. IP: HEK-293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| IP |
1/100 - 1/500.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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ChIP
Use at an assay dependent concentration. |
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IP
1/100 - 1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
Tissue specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
Involvement in disease
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
Sequence similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6935 Human
- Entrez Gene: 21417 Mouse
- Omim: 189909 Human
- SwissProt: P37275 Human
- SwissProt: Q64318 Mouse
- Unigene: 124503 Human
- Unigene: 3929 Mouse
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Alternative names
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
Images
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ChIP - Anti-ZEB1 antibody (ab155249)
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab155249. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9.
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Western blot - Anti-ZEB1 antibody (ab155249)All lanes : Anti-ZEB1 antibody (ab155249) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab155249)Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ZEB1 at nucleus (green) with ab155249 at 1/500 dilution. Red: Phalloidin, a cytoskeleton marker, at 1/100 dilution.
Scale bar = 10 μm.
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Immunoprecipitation - Anti-ZEB1 antibody (ab155249)IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
A - 40 μg of HEK-293T whole cell lysate.
B - Control with 2 μg of preimmune rabbit IgG.
C - Immunoprecipitation of ZEB1 protein by 2 μg of ab155249.
The immunoprecipitated ZEB1 protein was detected by ab155249 diluted at 1:1000, followed by anti-rabbit IgG.
7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab155249 has been referenced in 10 publications.
- Nappi A et al. The NANOG Transcription Factor Induces Type 2 Deiodinase Expression and Regulates the Intracellular Activation of Thyroid Hormone in Keratinocyte Carcinomas. Cancers (Basel) 12:N/A (2020). PubMed: 32197405
- Liu J et al. Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFß/SMAD2 axis in vitro. BMC Mol Cell Biol 21:65 (2020). PubMed: 32928102
- Wu T et al. Mir-30b-5p Promotes Proliferation, Migration, and Invasion of Breast Cancer Cells via Targeting ASPP2. Biomed Res Int 2020:7907269 (2020). PubMed: 32420372
- Chen YY et al. Protein arginine methyltranferase-1 induces ER stress and epithelial-mesenchymal transition in renal tubular epithelial cells and contributes to diabetic nephropathy. Biochim Biophys Acta Mol Basis Dis 1865:2563-2575 (2019). PubMed: 31199999
- Liu GM et al. Restoration of FBP1 suppressed Snail-induced epithelial to mesenchymal transition in hepatocellular carcinoma. Cell Death Dis 9:1132 (2018). PubMed: 30429463
- Wu T et al. Silencing of ASPP2 promotes the proliferation, migration and invasion of triple-negative breast cancer cells via the PI3K/AKT pathway. Int J Oncol 52:2001-2010 (2018). PubMed: 29568874
- Wang Z et al. Novel crosstalk between KLF4 and ZEB1 regulates gemcitabine resistance in pancreatic ductal adenocarcinoma. Int J Oncol 51:1239-1248 (2017). PubMed: 28849150
- Li S et al. Induction of epithelial-mesenchymal transition (EMT) by Beclin 1 knockdown via posttranscriptional upregulation of ZEB1 in thyroid cancer cells. Oncotarget 7:70364-70377 (2016). WB . PubMed: 27683118
- Yamada Y et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634
- Chen F et al. Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-ß signaling pathway. Mol Med Rep 10:1999-2003 (2014). PubMed: 25109410