Overview

  • Product name

  • Description

    Rabbit polyclonal to ZEB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ZEB1 aa 1074-1124.
    Database link: NP_110378.3

  • Positive control

    • IHC-P: Human breast carcinoma and prostate carcinoma tissues and mouse teratoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab87280 was affinity purified using an epitope specific to ZEB1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Function

    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
  • Tissue specificity

    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
  • Involvement in disease

    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
  • Sequence similarities

    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AREB 6 antibody
    • AREB6 antibody
    • BZP antibody
    • Delta crystallin enhancer binding factor 1 antibody
    • DELTA EF1 antibody
    • FECD6 antibody
    • MGC133261 antibody
    • Negative regulator of IL 2 antibody
    • Negative regulator of IL2 antibody
    • NIL 2 A antibody
    • NIL 2 A zinc finger protein antibody
    • NIL 2A antibody
    • NIL-2-A zinc finger protein antibody
    • NIL2A antibody
    • Posterior polymorphous corneal dystrophy 3 antibody
    • PPCD3 antibody
    • Represses interleukin 2 expression antibody
    • TCF 8 antibody
    • TCF-8 antibody
    • TCF8 antibody
    • Transcription factor 8 (represses interleukin 2 expression) antibody
    • Transcription factor 8 antibody
    • ZEB 1 antibody
    • ZEB antibody
    • ZEB1 antibody
    • ZEB1_HUMAN antibody
    • ZFHEP antibody
    • ZFHX 1A antibody
    • ZFHX1A antibody
    • Zinc finger E box binding homeobox 1 antibody
    • Zinc finger E-box-binding homeobox 1 antibody
    • Zinc finger homeodomain enhancer binding protein antibody
    see all

Images

  • ab87280, at 1/250 dilution, staining ZEB1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry. Detection: DAB staining.

  • ab87280, at 1/250 dilution, staining ZEB1 in formalin fixed, paraffin embedded mouse teratoma tissue by Immunohistochemistry. Detection: DAB staining.

References

This product has been referenced in:

  • Maleki S  et al. The mir-200 family regulates key pathogenic events in ascending aortas of individuals with bicuspid aortic valves. J Intern Med 285:102-114 (2019). Read more (PubMed: 30280445) »
  • Miyamoto M  et al. The Haphazard Pattern in Grade-3 Endometrioid Carcinoma Is Associated with Poor Prognosis and Tumor Lymphocyte Infiltration. Pathol Oncol Res N/A:N/A (2019). Read more (PubMed: 30810895) »
See all 10 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Dec 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Dec 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Dec 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tumor xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
tumor xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Dec 28 2018

Answer

Mi compañera de Servicio al Cliente me ha reenviado su consulta acerca del anticuerpo ab87280.Lamento mucho que el anticuerpo no haya funcionado tal y como esperaban. Todos nuestros productos están cubiertos por la garantía Abpromise, y en caso de que no funcionen como se especifica en la datasheet, estaremos encantados de ofrecer Soporte Técnico, y llegado el caso un reemplazo del vial o vale para una futura compra. Para tratar de entender mejor el problema, y monitorizar la calidad de nuestros productos, les agradecería mucho que me ampliaran la información del protocolo y las condiciones en las que se ha testado el producto, así como información referente al pedido y al lote concreto, para yo poder comprobar que no ha habido ninguna anomalía durante el envío ni con el lote concreto. Le mando un cuestionario de IHC, que se rellena en pocos minutos, y nos ayudará a entender por qué el anticuerpo no se ha comportado como se especifica en su ficha técnica. Una vez nos envíen completo el cuestionario, lo estudiaremos atentamente para poder ofrecerles una solución eficaz lo antes posible. No duden en contactarnos para cualquier otra duda o sugerencia.

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