RecombinantRabMAb

Recombinant Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488) (ab215964)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17375] to ZEB1 (Alexa Fluor® 488)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488)
    See all ZEB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17375] to ZEB1 (Alexa Fluor® 488)

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human ZEB1 aa 1-150. The exact sequence is proprietary.
    Database link: P37275

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17375

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.

  • Tissue specificity

    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.

  • Involvement in disease

    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.

  • Sequence similarities

    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • AREB 6 antibody
      • AREB6 antibody
      • BZP antibody
      • Delta crystallin enhancer binding factor 1 antibody
      • DELTA EF1 antibody
      • FECD6 antibody
      • MGC133261 antibody
      • Negative regulator of IL 2 antibody
      • Negative regulator of IL2 antibody
      • NIL 2 A antibody
      • NIL 2 A zinc finger protein antibody
      • NIL 2A antibody
      • NIL-2-A zinc finger protein antibody
      • NIL2A antibody
      • Posterior polymorphous corneal dystrophy 3 antibody
      • PPCD3 antibody
      • Represses interleukin 2 expression antibody
      • TCF 8 antibody
      • TCF-8 antibody
      • TCF8 antibody
      • Transcription factor 8 (represses interleukin 2 expression) antibody
      • Transcription factor 8 antibody
      • ZEB 1 antibody
      • ZEB antibody
      • ZEB1 antibody
      • ZEB1_HUMAN antibody
      • ZFHEP antibody
      • ZFHX 1A antibody
      • ZFHX1A antibody
      • Zinc finger E box binding homeobox 1 antibody
      • Zinc finger E-box-binding homeobox 1 antibody
      • Zinc finger homeodomain enhancer binding protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488) (ab215964)
      Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488) (ab215964)

      ab215964 staining ZEB1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215964 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
       
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488) (ab215964)
      Anti-ZEB1 antibody [EPR17375] (Alexa Fluor® 488) (ab215964)

    References (0)

    Publishing research using ab215964? Please let us know so that we can cite the reference in this datasheet.

    ab215964 has not yet been referenced specifically in any publications.

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