Key features and details
- Rabbit Polyclonal to ZEB2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZEB2 antibody
See all ZEB2 primary antibodies
DescriptionRabbit Polyclonal to ZEB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human cerebral cortex, glioma and colon tissues. WB: HEL cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 136 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
Involvement in diseaseDefects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
Sequence similaritiesBelongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain.
modificationsSumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
- Information by UniProt
- FLJ42816 antibody
- HSPC082 antibody
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Paraffin-embedded human cerebral cortex tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human glioma tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis. Negative control - no staining.
Anti-ZEB2 antibody (ab223688) at 1/100 dilution + HEL (human bone marrow erythroleukemia cell line) cell lysate
Predicted band size: 136 kDa
ab223688 has been referenced in 1 publication.
- Chen X et al. MicroRNA-30a regulates cell proliferation, migration, invasion and apoptosis in human nasopharyngeal carcinoma via targeted regulation of ZEB2. Mol Med Rep 20:1672-1682 (2019). PubMed: 31257481