ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
Involvement in disease
Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. Contains 7 C2H2-type zinc fingers. Contains 1 homeobox DNA-binding domain.
Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
Flow cytometric analysis of MDA-MB435 cells (green, right histogram) labeling Smad Interacting Protein 1 using ab170759 at a 1/10 dilution, compared with negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-Smad Interacting Protein 1 antibody - C-terminal (ab170759)
Anti-ZEB2 antibody - C-terminal (ab170759) at 1/100 dilution + MDA-MB435 cell lysate at 35 µg