Overview

  • Product name

    Anti-ZEB2 antibody [EPR21246]
    See all ZEB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21246] to ZEB2
  • Host species

    Rabbit
  • Specificity

    No signal was observed in Human colon FFPE sections.

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human bone marrow (FFPE), Human liver (FFPE), Hu glioma (FFPE)
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab230561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.01 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
  • Involvement in disease

    Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
  • Sequence similarities

    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ42816 antibody
    • HSPC082 antibody
    • KIAA0569 antibody
    • SIP 1 antibody
    • SIP1 antibody
    • Smad Interacting Protein 1 antibody
    • Smad-interacting protein 1 antibody
    • SMADIP 1 antibody
    • SMADIP1 antibody
    • ZEB 2 antibody
    • Zeb2 antibody
    • ZEB2_HUMAN antibody
    • Zfhx1b antibody
    • ZFHX1B protein antibody
    • Zfx1b antibody
    • Zinc finger E box binding protein 2 antibody
    • Zinc finger E-box-binding homeobox 2 antibody
    • Zinc finger homeobox 1b antibody
    • zinc finger homeobox protein 1 antibody
    • Zinc finger homeobox protein 1b antibody
    see all

Images

  • IHC image of ZEB2 staining in human bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230561, 0.01µg/ml, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of ZEB2 staining in human glioma formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230561, 0.01µg/ml, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of ZEB2 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230561, 0.01µg/ml, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab230561 has not yet been referenced specifically in any publications.

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