Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1] (ab73643)
Key features and details
- Mouse monoclonal [FIS 4E8/1] to Zebrafish Gut Absorptive Cell Epitopes
- Suitable for: IP, ICC/IF, IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1] -
Description
Mouse monoclonal [FIS 4E8/1] to Zebrafish Gut Absorptive Cell Epitopes -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IF, IHC - Wholemount, WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Tissue, cells or virus corresponding to Zebrafish Zebrafish Gut Absorptive Cell Epitopes. Lysate of zebrafish intestines.
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General notes
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells. -
Clonality
Monoclonal -
Clone number
FIS 4E8/1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24504339
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IHC - Wholemount |
Use at an assay dependent concentration. PubMed: 23352790
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WB |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24504339 |
IHC - Wholemount
Use at an assay dependent concentration. PubMed: 23352790 |
WB
Use at an assay dependent concentration. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab73643 has been referenced in 5 publications.
- Levic DS et al. Distinct roles for luminal acidification in apical protein sorting and trafficking in zebrafish. J Cell Biol 219:N/A (2020). IP ; Zebrafish . PubMed: 32328632
- Park J et al. Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. Dev Cell 51:7-20.e6 (2019). PubMed: 31474562
- Murdoch CC et al. Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization. PLoS Pathog 15:e1007381 (2019). PubMed: 30845179
- Alvers AL et al. Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Development 141:1110-9 (2014). ICC/IF ; Zebrafish . PubMed: 24504339
- Crosnier C et al. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development 132:1093-104 (2005). PubMed: 15689380