Overview

  • Product name

    Anti-ZF21 antibody - N-terminal
  • Description

    Rabbit polyclonal to ZF21 - N-terminal
  • Host species

    Rabbit
  • Specificity

    At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ZF21 (N terminal). The exact sequence is proprietary. 15 amino acid peptide.
    Database link: Q9BQ24

  • Positive control

    • Human kidney tissue. 3T3 cell lysate.
  • General notes

    Previously labelled as ZFYVE21. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Plays a role in cell adhesion, and thereby in cell motility, regulating microtubule-induced PTK2/FAK1 dephosphorylation, an event important for focal adhesion disassemblya, as well as integrin beta-1/ITGB1 cell surface expression.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
  • Cellular localization

    Cell junction > focal adhesion. Cytoplasmic vesicle. Within cytoplasmic vesicles, partially colocalizes with EEA1, an endosomal marker.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110013H04Rik antibody
    • AW558780 antibody
    • C85416 antibody
    • FYVE domain containing 21 antibody
    • MGC2550 antibody
    • ZF21 antibody
    • ZFY21_HUMAN antibody
    • ZFYVE 21 antibody
    • ZFYVE21 antibody
    • Zinc finger FYVE domain containing 21 antibody
    • Zinc finger FYVE domain containing protein 21 antibody
    • Zinc finger FYVE domain-containing protein 21 antibody
    see all

Images

  • All lanes : Anti-ZF21 antibody - N-terminal (ab189935) at 1 µg/ml

    Lane 1 : 3T3 cell lysate
    Lane 2 : 3T3 cell lysate plus blocking peptide

    Predicted band size: 27 kDa

  • Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue sections labeling ZF21 with ab189935 at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence analysis of human kidney cells labeling ZF21 using ab189935 at 20 µg/ml.

References

ab189935 has not yet been referenced specifically in any publications.

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