Overview

  • Product name
  • Description
    Rabbit polyclonal to ZFAND3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within Human ZFAND3 aa 38-87 (N terminal). The exact sequence is proprietary.
    Sequence:

    DFQKKQPDDDSAPSTSNSQSDLFSEETTSDNNNTSITTPTLSPSQQPLPT


    Database link: Q9H8U3

  • Positive control
    • 721B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-ZFAND3 antibody (ab80396) at 1 µg/ml + 721B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

References

ab80396 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80396.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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