Overview

  • Product name
    Anti-Zfp219 antibody - Aminoterminal end
    See all Zfp219 primary antibodies
  • Description
    Rabbit polyclonal to Zfp219 - Aminoterminal end
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 161-191 of Human Zfp219

  • Positive control
    • 293 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 30, 55, 60, 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

Images

  • Anti-Zfp219 antibody - Aminoterminal end (ab71279) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 77 kDa
    Observed band size: 77 kDa
    Additional bands at: 30 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • ab71279 at 1/50 dilution staining Zfp219 in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

This product has been referenced in:
  • Takigawa Y  et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J Cell Sci 123:3780-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20940257) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

I understand that you have a deadline coming up quickly and I really wish I could hurry the production process along. We do make every effort to produce the antibodies as quickly as possible, as you can imagine we can't sell the antibodies if they are not in stock. Unfortunately the production process is months long and already as streamlined as possible.\ If you wish you can set an alert that will send you an email when the antibody is in stock. Simply go to the online datasheet for ab31003 https://www.abcam.com/Zfp219-antibody-ab31003.htmland click on the word "Login" in the blue'Availability' section at the top of the datasheet where it states "https://www.abcam.com/account/home to be notified of stock". I'm sorry I could not be of further help in this instance. Please let me know if you have any other questions.

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Answer

Thanks for sending along the western blot and IHC data. That was very helpful. I agree it is odd that the same 2 intense bands are consistantly detected in all your blots. As well, the IHC staining should be largely nuclear and it is not. And, as you said, the recombinant znf219 is detected with the anti-V5 tag antibody but not with ab71279. Based on all this data itseems doubtful that the˜70kDa band seen in your blots is actually znf219. I checked our stocks and at this time we only have the same lot of ab71279 available. We do have another anti-znf219 antibody, ab31003, although currently back ordered and not scheduled for release until at least mid-February. In this case I would be happy to send you the remaining vial of ab71279, even though it is of the same lot, or I could offer a credit or refund.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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