Overview

  • Product name

    Anti-Zfp281/ZNF281 antibody
    See all Zfp281/ZNF281 primary antibodies
  • Description

    Rabbit polyclonal to Zfp281/ZNF281
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Zfp281/ZNF281 aa 800 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab130905)

  • Positive control

    • This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281/ZNF281 null mouse embryonic stem cell lysate.
  • General notes

     This product was previously labelled as Zfp281

     

Properties

Applications

Our Abpromise guarantee covers the use of ab101318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • GC box binding zinc finger protein 1 antibody
    • GC-box-binding zinc finger protein 1 antibody
    • GZP1 antibody
    • Transcription factor ZBP-99 antibody
    • ZBP 99 antibody
    • ZBP99 antibody
    • zfp281 antibody
    • Zinc finger DNA binding protein 99 antibody
    • Zinc finger DNA-binding protein 99 antibody
    • Zinc finger protein 281 antibody
    • ZN281_HUMAN antibody
    • ZNF281 antibody
    • ZNP 99 antibody
    • ZNP 99 transcription factor antibody
    • ZNP99 antibody
    see all

Images

  • All lanes : Anti-Zfp281/ZNF281 antibody (ab101318) at 1 µg/ml

    Lane 1 : wild type mouse embryonic stem cell lysate
    Lane 2 : Zfp281/ZNF281 null mouse embryonic stem cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Zfp281/ZNF281 was immunoprecipitated using 2µg/ml of ab101318 and 2mg of wild type Mouse embryonic stem cell nuclear extract (lane 1). Mouse embryonic stem cells negative for Zfp281/ZNF281 were used as a negative control (lane 2). Band at ~98kDa is Zfp281/ZNF281.

    See Abreview

References

This product has been referenced in:

  • Huang X  et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. Elife 6:N/A (2017). Read more (PubMed: 29168693) »
  • Fidalgo M  et al. Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States. Cell Stem Cell 19:355-69 (2016). Read more (PubMed: 27345836) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Total protein in input
2000 µg
Specification
mouse embryonic stem cells
Immuno-precipitation step
Protein G

Mr. Miguel Fidalgo

Verified customer

Submitted May 04 2012

Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Miguel Fidalgo

Verified customer

Submitted May 04 2012

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