Western blot abreview for Anti-ZNF342 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (Multiple cell types)
Loading amount
30 µg
Specification
Multiple cell types
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Concentration
1 µg/ml

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 48 kDa Non-specific: 75 kDa
Positive control
nuclear extracts from human cell lines
Negative control
none

Additional data

Additional Notes
30ug of nuclear extract from four human cell lines were tested for the expression of ZNF342. The ZNF342 protein has an expected band size approximately 50kDa, which was detected using the ab51265 antibody.

Dr. Seth Frietze

Verified customer

Submitted Jun 30 2011

Sign up