Recombinant Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19926] to ZFP36L1 (phospho S92)
- Suitable for: Dot blot, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ZFP36L1 (phospho S92) antibody [EPR19926]
See all ZFP36L1 primary antibodies -
Description
Rabbit monoclonal [EPR19926] to ZFP36L1 (phospho S92) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate; Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate. Dot blot: ZFP36L1 (phospho S92) peptide (aa87-97).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19926 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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WB |
1/5000. Detects a band of approximately 36-47 kDa (predicted molecular weight: 36 kDa).
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Notes |
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Dot blot
1/1000. |
WB
1/5000. Detects a band of approximately 36-47 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Probable regulatory protein involved in regulating the response to growth factors. -
Sequence similarities
Contains 2 C3H1-type zinc fingers. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 677 Human
- Entrez Gene: 12192 Mouse
- Omim: 601064 Human
- SwissProt: Q07352 Human
- SwissProt: P23950 Mouse
- Unigene: 707091 Human
- Unigene: 85155 Human
- Unigene: 235132 Mouse
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Alternative names
- Berg36 antibody
- BRF1 antibody
- Butyrate response factor 1 antibody
see all
Images
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All lanes : Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/2500 dilution
Lane 1 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane)
Lane 2 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 3 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane)
Lane 4 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane)
Lane 6 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 7 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 8 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 9 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 10 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, (Phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773); and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/15000 dilution
Predicted band size: 36 kDa
Observed band size: 36-47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST.
Lysates from the ZFP36L1fl/fl CD4 cre KO mouse were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.
Lysates used for the WB were isolated by negative selection using biotinylated abs against Ter119, CD11b, CD11c, Gr1, CD19, B220, F4/80 and NK1.1 to leave a population of CD4 and CD8 positive T cells (the purity of the population was >95% as checked by flow).
Mouse anti-vinculin ab130007, used as the loading control.
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All lanes : Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, then treated with alkaline phosphatase for 1 hour on the membrane
Lane 4 : HEK-293T transfected with human ZFP36L1 S92A mutant expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 36-47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 2% BSA/TBST.
All plasmids were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.
The expression profile observed is consistent with what has been described in the literature (PMID: 17030608).
Exposure time: 8 seconds
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Dot blot analysis of ZFP36L1 (phospho S92) labeled with ab204922 at 1/1000 dilution.
Lane 1: ZFP36L1 (phospho S92) peptide (aa87-97).
Lane 2: ZFP36L1 non-phospho peptide (aa87-97).
Lane 3: ZFP36L1 (phospho S125) peptide (aa120-130).
Lane 4: ZFP36L1 non-phospho peptide (aa120-130).Blocking and dilution buffer: 5% NFDM/TBST.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab204922 has been referenced in 1 publication.
- Feng H et al. MALAT1 Induces Food Allergy by Promoting Release of IL-6 from Dendritic Cells and Suppressing the Immunomodulatory Function of Tregs. J Asthma Allergy 15:529-544 (2022). PubMed: 35515816