Product nameAnti-Zfp57 antibody
See all Zfp57 primary antibodies
DescriptionRabbit polyclonal to Zfp57
Tested applicationsSuitable for: ChIP, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
- This antibody gave a positive signal in Wt mouse ES cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab45341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 26728555|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).|
RelevanceZfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
- bA145L22 antibody
- bA145L22.2 antibody
- C6orf40 antibody
All lanes : ab45341 at 10 µg
Lane 1 : Wild-type Mouse ES cell lysate
Lane 2 : Mouse Zfp57 KO cell lysate
Lane 3 : Mouse ES-E14TG2a whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 10 kDa (possible non-specific binding), 149 kDa (possible non-specific binding), 98 kDa (possible non-specific binding)
Zfp57 KO ES cell lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, University of Cambridge. The mutant mouse used to make these lysates is described here - Takahashi et al., Cold Spring Harb Symp Quant Biol. 2015;80:177-87.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab45341 and anti-GAPDH as a loading control (shown in Red) overnight at 4°C. Antibody binding was detected using ab216773 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This product has been referenced in:
- Matsuzaki H et al. Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice. Epigenetics Chromatin 11:36 (2018). Read more (PubMed: 29958543) »
- Coluccio A et al. Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells. Epigenetics Chromatin 11:7 (2018). Read more (PubMed: 29482634) »