• Product name

  • Description

    Rabbit polyclonal to Zfp57
  • Host species

  • Tested applications

    Suitable for: ChIP, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Zfp57 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab45340)

  • Positive control

    • This antibody gave a positive signal in wild type mouse ES cell lysates.



Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 26728555
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).


  • Relevance

    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localization

  • Database links

  • Alternative names

    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all


  • All lanes : Anti-Zfp57 antibody (ab45341) at 1/1000 dilution

    Lanes 1-2 : Wild type mouse embryonic stem cell lysate
    Lane 3 : Zfp57 knockout mouse embryonic stem cell lysate

    All lanes : Goat anti Rabbit HRP at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 48 kDa


This product has been referenced in:

  • Matsuzaki H  et al. Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice. Epigenetics Chromatin 11:36 (2018). Read more (PubMed: 29958543) »
  • Coluccio A  et al. Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells. Epigenetics Chromatin 11:7 (2018). Read more (PubMed: 29482634) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for contacting us. No, these antibodies have not been tested with overexpression or knockdown cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for contacting us.

Our testing did show a band at this range in mouse brain lysate as well (https://www.abcam.com/Zfp57-antibody-ab45341.html#ab45341_1.jpg) that we were not able to identify. To our knowledge, this product has not previously been tested in rat however taking into account the appearance of a band at this weight in our mouse data, this effect is not surprising.

We do carry the peptide for this antibody (https://www.abcam.com/zfp57-peptide-ab45340.html)should you wish to perform an immunogen blocking procedure. Our recommended protocol for immunogen blocking can be found at this address:https://www.abcam.com/zfp57-peptide-ab45340.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab did not get satisfactory results in ChIP. Unfortunately we currently do not have any other anti-Human Zfp57 in the development pipeline. However, if you want to test this antibody in ChIP, I can offer a discount off a future purchase if you buy ab50944 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab50944 in ChIP. I will then send a discount code. This code must be issued before purchasing ab50944 so please wait for my reply before ordering. 2. Purchase ab50944 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab50944 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Mouse Tissue lysate - whole (Testes)
Total protein in input
20 µg
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Aug 02 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Apr 01 2008

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