Anti-Zfp57 antibody (ab45341)
Key features and details
- Rabbit polyclonal to Zfp57
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Zfp57 antibody -
Description
Rabbit polyclonal to Zfp57 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse Zfp57 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45340) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa). |
Target
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Relevance
Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 22715 Mouse
- Entrez Gene: 361783 Rat
- SwissProt: Q8C6P8 Mouse
- SwissProt: A0JPK3 Rat
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Alternative names
- bA145L22 antibody
- bA145L22.2 antibody
- C6orf40 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab45341 has been referenced in 17 publications.
- Zhang H et al. Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells. Protein Cell 13:102-119 (2022). PubMed: 34865203
- LeBlanc L et al. β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience 25:103541 (2022). PubMed: 34977504
- Kojima S et al. Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain. Nucleic Acids Res 50:5080-5094 (2022). PubMed: 35544282
- Acurzio B et al. The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex. Epigenetics Chromatin 15:27 (2022). PubMed: 35918739
- Hara S et al. Humanization of a tandem repeat in IG-DMR causes stochastic restoration of paternal imprinting at mouse Dlk1-Dio3 domain. Hum Mol Genet 30:564-574 (2021). PubMed: 33709141