Key features and details
- Rabbit polyclonal to Zfp57
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
RelevanceZfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
- bA145L22 antibody
- bA145L22.2 antibody
- C6orf40 antibody
Anti-Zfp57 antibody (ab50944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50,000
Predicted band size: 59 kDa
Observed band size: 64,65,66 kDa why is the actual band size different from the predicted?
Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.
ab50944 has not yet been referenced specifically in any publications.