• Product name

  • Description

    Rabbit polyclonal to Zfp57
  • Host species

  • Specificity

    This antibody reacts with Zfp57
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: MFEQLKPIEP RDCWREARVK KKPVTFEDVA VNFTQEEWDC LDASQRVLYQ, corresponding to N terminal amino acids 1-50 of Human Zfp57

  • Positive control

    • WB: Jurkat cell lysate IHC-P: Human kidney tissue IF/ICC: MEF1 cell line.



Our Abpromise guarantee covers the use of ab50944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • Relevance

    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localization

  • Database links

  • Alternative names

    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all


  • ICC/IF image of ab50944 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50944, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Zfp57 antibody (ab50944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50,000

    Predicted band size: 59 kDa
    Observed band size: 64,65,66 kDa
    why is the actual band size different from the predicted?

  • Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.


ab50944 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Blocking step
Protein Block (Leica) as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 21°C

Ms. Eva Rodriguez Nunez

Verified customer

Submitted Aug 05 2015


Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab did not get satisfactory results in ChIP. Unfortunately we currently do not have any other anti-Human Zfp57 in the development pipeline. However, if you want to test this antibody in ChIP, I can offer a discount off a future purchase if you buy ab50944 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab50944 in ChIP. I will then send a discount code. This code must be issued before purchasing ab50944 so please wait for my reply before ordering. 2. Purchase ab50944 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab50944 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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