Overview

  • Product name
    Anti-ZFPL1 antibody
  • Description
    Rabbit polyclonal to ZFPL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide derived from a region within residues 101-150 of human ZFPL1.

    Read Abcam's proprietary immunogen policy .

  • Positive control
    • HepG2 cell lysate for Western blot and human kidney tissue for Immunohistochemistry.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    ZFPL1 is a regulatory transcription factor that is expressed strongly in the exocrine pancreas. It contains 1 RING type zinc finger. It is required for cis-Golgi integrity and efficient ER to Golgi transport. Involved in the maintainance of the integrity of the cis-Golgi, possibly via its interaction with GOLGA2/GM130.
  • Cellular localization
    Golgi Apparatus and Nuclear; Single-pass membrane protein.
  • Database links
  • Alternative names
    • D11S750 antibody
    • MCG4 antibody
    • MGC 4 antibody
    • ZFPL 1 antibody
    • Zinc finger protein like 1 antibody
    • Zinc finger protein MCG4 antibody
    • zinc-finger protein in MEN1 region antibody
    see all

Images

  • Anti-ZFPL1 antibody (ab26057) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate 10ug.

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Ab26057 at 4-8 ug/ml staining ZFPL1 in paraffin embedded human kidney tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of the renal tube.

References

This product has been referenced in:
  • Cai MJ  et al. RING domain of zinc finger protein like 1 is essential for cell proliferation in endometrial cancer cell line RL95-2. Gene 677:17-23 (2018). Read more (PubMed: 30036656) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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