Product nameAnti-Zhangfei antibody
See all Zhangfei primary antibodies
DescriptionRabbit polyclonal to Zhangfei
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
- This antibody gave a positive signal in the following whole cell lysates: 293T Embryonic Kidney cell line (Human), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), HeLa (Human epithelial carcinoma cell line), PC3 (Human prostate cancer cell line), NIH 3T3 (Mouse embryonic fibroblast cell line). This antibody also gave a positive signal in the following tissue lysates: Human Heart, Human Brain, Human Kidney, Human Liver.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).|
FunctionStrongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
Tissue specificityIn adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Cellular localizationNucleus. Colocalizes at punctate nuclear structures with CREB3 and HCFC1.
- Information by UniProt
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All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 :
PC3 whole cell lysate (ab3954)
Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.
This product has been referenced in:
- Chen F et al. Construction and expression of lentiviral vectors encoding recombinant mouse CREBZF in NIH 3T3 cells. Plasmid 76C:24-31 (2014). Read more (PubMed: 25195838) »
- López-Mateo I et al. The transcription factor CREBZF is a novel positive regulator of p53. Cell Cycle 11:3887-95 (2012). Read more (PubMed: 22983008) »