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Why does this antibody detect a doublet around 50 kDa? The other Zhangfei antibodies in your catalog detect bands of different molecular weights, and the predicted molecular weight of this protein is 38 kDa. Are there multiple isoforms of Zhangfei? What is this antibody detecting?
Asked on Oct 18 2011
Further to my previous message, our lab has retested this antibody and produced the attached results. Ab28700 primarily detects the Smile-L isoform. I hope this helps, please let me know if you need any additional information. All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431) Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate (ab106304) Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab52257) Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate (ab28419) Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) Lane 9 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954) Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab52956) Lysates/proteins at 20 µg per lane. Secondary Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution Performed under reducing conditions. Predicted band size : 30 kDa Exposure time : 2 minutes
Answered on Oct 18 2011