Anti-ZHX1 antibody (ab19356)
Key features and details
- Rabbit polyclonal to ZHX1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZHX1 antibody
See all ZHX1 primary antibodies -
Description
Rabbit polyclonal to ZHX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig, Chimpanzee, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ZHX1 aa 1-50.
Database link: 11244 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa).
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa). |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acts as a transcriptional repressor. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the ZHX family.
Contains 2 C2H2-type zinc fingers.
Contains 5 homeobox DNA-binding domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11244 Human
- Entrez Gene: 22770 Mouse
- Omim: 604764 Human
- SwissProt: A2T771 Chimpanzee
- SwissProt: Q9UKY1 Human
- SwissProt: P70121 Mouse
- SwissProt: A2T7S4 Orangutan
- Unigene: 612084 Human
see all -
Alternative names
- ZHX 1 antibody
- ZHX1 antibody
- ZHX1_HUMAN antibody
see all
Images
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All lanes : Anti-ZHX1 antibody (ab19356) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCML-1 whole cell lysate
Lane 5 : NIH-3T3 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 100 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling ZHX1 with ab19356 at 1/1000 (1µg/ml). Detection: DAB.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab19356 has been referenced in 2 publications.
- Mondal S et al. PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Nat Methods 19:959-968 (2022). PubMed: 35927480
- Chen S et al. The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity. J Cell Biochem 114:2323-33 (2013). PubMed: 23686912