Anti-ZHX2 antibody (ab96083)
Key features and details
- Rabbit polyclonal to ZHX2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZHX2 antibody
See all ZHX2 primary antibodies -
Description
Rabbit polyclonal to ZHX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, containing a sequence corresponding to a region within amino acids 533 and 796 of Human ZHX2 (NP_055758)
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Positive control
- A431 cells (IF), H1299, HeLa, Molt-4, Raji cell lysate. HepG2 whole cell lysate. SAS Xenograft (IHC)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 92 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Acts as a transcriptional repressor. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the ZHX family.
Contains 2 C2H2-type zinc fingers.
Contains 4 homeobox DNA-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22882 Human
- Omim: 609185 Human
- SwissProt: Q9Y6X8 Human
- Unigene: 377090 Human
- Unigene: 658443 Human
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Alternative names
- AFP regulator 1 antibody
- AFR1 antibody
- Alpha fetoprotein regulator 1 antibody
see all
Images
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Anti-ZHX2 antibody (ab96083) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 92 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96083 at 1/200 dilution. (1) ab96083, (2) Merged with DNA probe
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Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ab96083 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96083 has been referenced in 1 publication.
- Creasy KT et al. Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver. Gene Expr 17:7-17 (2016). IHC-Fr ; Mouse . PubMed: 27197076