Product nameAnti-ZHX3 antibody
See all ZHX3 primary antibodies
DescriptionRabbit polyclonal to ZHX3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human ZHX3 (NP_055850.1)
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease.
Tissue specificityWidely expressed. High expression in kidney.
Sequence similaritiesBelongs to the ZHX family.
Contains 2 C2H2-type zinc fingers.
Contains 5 homeobox DNA-binding domains.
- Information by UniProt
- KIAA0395 antibody
- TIX1 antibody
- Triple homeobox 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99350 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-ZHX3 antibody (ab99350) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. ab99350 used for WB at 1 ug/ml and used for IP at 3 ug/mg lysate (lane 1). ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 10 seconds.
ab99350 has not yet been referenced specifically in any publications.