Overview

  • Product name

  • Description

    Rabbit polyclonal to Zic1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Zic1 (internal sequence). The exact sequence is proprietary.
    Database link: Q15915

  • Positive control

    • WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.

Target

  • Function

    Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
  • Tissue specificity

    CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain

    The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • Cellular localization

    Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression.
  • Information by UniProt
  • Database links

  • Alternative names

    • Odd paired homolog Drosophila antibody
    • Zic 1 antibody
    • ZIC antibody
    • Zic family member 1 (odd-paired Drosophila homolog) antibody
    • Zic family member 1 antibody
    • Zic protein member 1 antibody
    • zic1 antibody
    • ZIC1_HUMAN antibody
    • Zinc finger protein 201 antibody
    • Zinc finger protein of the cerebellum 1 antibody
    • Zinc finger protein ZIC 1 antibody
    • Zinc finger protein ZIC1 antibody
    • ZNF 201 antibody
    • ZNF201 antibody
    see all

Images

  • All lanes : Anti-Zic1 antibody (ab229262) at 1/500 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
    Lane 3 : IMR32 whole cell lysate
    Lane 4 : SK-N-AS whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 48 kDa



    10% SDS-PAGE

References

ab229262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229262.
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