Recombinant
RabMAb

Recombinant Anti-Zic1 antibody [EPR7291(2)] (ab134951)

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Overview

  • Product name

    Anti-Zic1 antibody [EPR7291(2)]
    See all Zic1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7291(2)] to Zic1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A synthetic peptide corresponding to residues within Human Zic1 (UniProt ID: Q15915).

  • Positive control

    • Human cerebellum lysate, Human brain, Human fetal brain lysate, SH SY5Y cell lysate, SW480 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab134951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.Can be blocked with Zic1 peptide (ab201520).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/1000.

 

Target

  • Function

    Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.

  • Tissue specificity

    CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.

  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.

  • Domain

    The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

  • Cellular localization

    Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression.
  • Information by UniProt

  • Database links

    • Alternative names

      • Odd paired homolog Drosophila antibody
      • Zic 1 antibody
      • ZIC antibody
      • Zic family member 1 (odd-paired Drosophila homolog) antibody
      • Zic family member 1 antibody
      • Zic protein member 1 antibody
      • zic1 antibody
      • ZIC1_HUMAN antibody
      • Zinc finger protein 201 antibody
      • Zinc finger protein of the cerebellum 1 antibody
      • Zinc finger protein ZIC 1 antibody
      • Zinc finger protein ZIC1 antibody
      • ZNF 201 antibody
      • ZNF201 antibody
      see all

    Images

    • Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/2000 dilution (purified)

      Lane 1 : Human brain lysates
      Lane 2 : Human cerebellum lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Zic1 with purified ab134951 at 1:70 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Zic1  with Purified ab134951 at 1:200 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)

      Overlay histogram showing SH-SY5Y cells stained with unpurified ab134951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/1000 dilution (unpurified)

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : SH SY5Y cell lysate
      Lane 4 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 48 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
      Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)

      Immunofluorescent staining of SW480 cells labelling Zic1 with unpurified ab134951 at 1/100 dilution

    References

    This product has been referenced in:

    • Wang X  et al. Evaluation and optimization of differentiation conditions for human primary brown adipocytes. Sci Rep 8:5304 (2018). Read more (PubMed: 29593245) »
    • Rheaume BA  et al. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nat Commun 9:2759 (2018). Read more (PubMed: 30018341) »
    See all 4 Publications for this product

    Publishing research using ab134951? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Zic1 antibody [EPR7291(2)]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis)
    Permeabilization
    Yes - Triton X-100
    Specification
    Testis
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 03 2020

    ChIP abreview for Anti-Zic1 antibody [EPR7291(2)]

     Poor
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Metastatic melanoma cell line)
    Negative control
    ChIP-sequencing
    Specification
    Metastatic melanoma cell line
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Double crosslinking. 45min in
    Positive control
    ChIP-sequencing
    Read More

    Mr. Dan Hasson

    Verified customer

    Submitted Dec 10 2018

    Western blot abreview for Anti-Zic1 antibody [EPR7291(2)]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (sucutaneous adipose tissue)
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Loading amount
    20 µg
    Specification
    sucutaneous adipose tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Zic1 antibody [EPR7291(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
    Sample
    Mouse Tissue sections (mouse cerebellum)
    Specification
    mouse cerebellum
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2015

    Western blot abreview for Anti-Zic1 antibody [EPR7291(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (mouse cerebellum)
    Specification
    mouse cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2014

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