- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Zic2 antibody
See all Zic2 primary antibodies -
DescriptionRabbit polyclonal to Zic2
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Host speciesRabbit
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SpecificityRecognises a band at about 60kD in mouse brain lysate (but not in rat brain lysate). A lower band is also detected, the identity of this is unknown.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Cow, Human, Zebrafish
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Immunogen
Synthetic peptide:
GRSEQYRQVASPRTDPYS
conjugated to KLH, corresponding to amino acids 190-207 of Mouse Zic2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab10550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
Target
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FunctionActs as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
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Involvement in diseaseDefects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
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Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
DomainThe C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
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Post-translational
modificationsPhosphorylated.
Ubiquitinated by RNF180, leading to its degradation. -
Cellular localizationNucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 514433 Cow
- Entrez Gene: 7546 Human
- Entrez Gene: 22772 Mouse
- Omim: 603073 Human
- SwissProt: O95409 Human
- SwissProt: Q62520 Mouse
- Unigene: 653700 Human
- Unigene: 308936 Mouse
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Alternative names
- HPE 5 antibody
- HPE5 antibody
- Odd paired homolog Drosophila antibody
see all
Images
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Western blot - Anti-Zic2 antibody (ab10550)Western blot using ab10550 at 1/500.
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Rat Brain tissue lysateThe upper band in the mouse brain lysate represents Zic2.
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 5 minutes
Expected molecular weight: 55 kDa
20µ Western blot using ab10550 at 1/500.
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Rat Brain tissue lysateThe upper band in the mouse brain lysate represents Zic2.
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 5 minutes
Expected molecular weight: 55 kDa
20µg of cell lysate per lane
Protocols
References
This product has been referenced in:
- Glendining KA et al. Downstream mediators of Ten-m3 signalling in the developing visual pathway. BMC Neurosci 18:78 (2017). Read more (PubMed: 29207951) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"