• Product name

  • Description

    Goat polyclonal to Zic2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

  • Specificity

    ab121036 is not expected to cross-react with other Zic proteins.
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:


    by a Cysteine residue linker, corresponding to C terminal amino acids 520-531 of Human Zic2 (NP_009060.2).


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab121036 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide
  • Clonality

  • Isotype

  • Research areas


Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates .


  • Function

    Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
  • Involvement in disease

    Defects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain

    The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • Post-translational

    Ubiquitinated by RNF180, leading to its degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HPE 5 antibody
    • HPE5 antibody
    • Odd paired homolog Drosophila antibody
    • Zic 2 antibody
    • Zic family member 2 (odd paired Drosophila homolog) antibody
    • Zic family member 2 antibody
    • ZIC2 antibody
    • ZIC2_HUMAN antibody
    • Zinc finger protein of the cerebellum 2 antibody
    • Zinc finger protein ZIC 2 antibody
    • Zinc finger protein Zic2 antibody
    see all


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with ab121036 (0.5ug/ml) in the right panel(B) and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel (A). Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.


ab121036 has not yet been referenced specifically in any publications.

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