Overview

  • Product name

  • Description

    Goat polyclonal to ZIC3
  • Host species

    Goat
  • Specificity

    ab133153 is NOT expected to cross-react with other ZIC proteins.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-HVDNNQVHLGLR

    with a Cysteine residue linker, corresponding to internal sequence amino acids 178-189 of Human ZIC3 NP_003404.1.

Properties

Applications

Our Abpromise guarantee covers the use of ab133153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. Preliminary experiments gave an approx 70kDa band in Human, Muse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kDa according to NP_003404.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.

Target

  • Function

    Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
  • Involvement in disease

    Heterotaxy, visceral, 1, X-linked
    VACTERL association X-linked with or without hydrocephalus
    Congenital heart defects, multiple types, 1, X-linked
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain

    The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • Cellular localization

    Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heterotaxy 1 antibody
    • HTX antibody
    • HTX1 antibody
    • VACTERLX antibody
    • ZIC 3 antibody
    • Zic family member 3 (odd paired Drosophila homolog heterotaxy 1) antibody
    • Zic family member 3 antibody
    • Zic3 antibody
    • ZIC3_HUMAN antibody
    • Zinc finger protein 203 antibody
    • Zinc finger protein of the cerebellum 3 antibody
    • Zinc finger protein ZIC 3 (Zinc finger protein of the cerebellum 3) antibody
    • Zinc finger protein ZIC 3 antibody
    • ZNF203 antibody
    see all

Images

  • ab133153 (5µg/ml) showsstaining ZOC3 in paraffin embedded Human Prostate following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows nuclear staining in select secretory cells.

References

ab133153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Differentiated human embryonic stem cells)
Permeabilization
Yes - PBS/0.1% Triton X-100
Specification
Differentiated human embryonic stem cells
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Dec 23 2016

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