Key features and details
- Goat polyclonal to ZIC3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZIC3 antibody
See all ZIC3 primary antibodies
DescriptionGoat polyclonal to ZIC3
Specificityab133153 is NOT expected to cross-react with other ZIC proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab133153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Involvement in diseaseHeterotaxy, visceral, 1, X-linked
VACTERL association X-linked with or without hydrocephalus
Congenital heart defects, multiple types, 1, X-linked
Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
DomainThe C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
Cellular localizationNucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.
- Information by UniProt
- Heterotaxy 1 antibody
- HTX antibody
- HTX1 antibody
ab133153 has not yet been referenced specifically in any publications.