Anti-ZIC3 antibody (ab215063)
Key features and details
- Sheep polyclonal to ZIC3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-ZIC3 antibody
See all ZIC3 primary antibodies -
Description
Sheep polyclonal to ZIC3 -
Host species
Sheep -
Specificity
The immunogen for this antibody shares 85% homology with Zic2 and 73% homology with Zic1. This antibody is expected to detect both Zic1 and Zic2 as well as Zic3.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Recombinant fragment corresponding to Human ZIC3 aa 350 to the C-terminus. (Dervied from E.coli).
Database link: O60481 -
Positive control
- Mouse embryonic tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The IgG fraction of immunized serum was purified by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. -
Involvement in disease
Heterotaxy, visceral, 1, X-linked
VACTERL association X-linked with or without hydrocephalus
Congenital heart defects, multiple types, 1, X-linked -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Domain
The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation. -
Cellular localization
Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6. - Information by UniProt
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Database links
- Entrez Gene: 7547 Human
- Entrez Gene: 22773 Mouse
- Omim: 300265 Human
- SwissProt: O60481 Human
- SwissProt: Q62521 Mouse
- Unigene: 111227 Human
- Unigene: 255890 Mouse
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Alternative names
- Heterotaxy 1 antibody
- HTX antibody
- HTX1 antibody
see all
Images
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Anti-ZIC3 antibody (ab215063) at 1/1000 dilution + Mouse embryonic tissue lysate
Predicted band size: 50 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab215063 has been referenced in 2 publications.
- Chen D et al. LncRNA IGBP1-AS1/miR-24-1/ZIC3 loop regulates the proliferation and invasion ability in breast cancer. Cancer Cell Int 20:153 (2020). PubMed: 32390766
- Yamanishi A et al. Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis. Nucleic Acids Res 46:e63 (2018). PubMed: 29554276