Anti-ZIC3 antibody (ab222124)
Key features and details
- Rabbit polyclonal to ZIC3
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZIC3 antibody
See all ZIC3 primary antibodies -
Description
Rabbit polyclonal to ZIC3 -
Host species
Rabbit -
Specificity
Based on the homology of immunogen sequence, this antibody may cross-react with other proteins of ZIC family. Please note that this has not been experimentally tested.
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Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human ZIC3 aa 400 to the C-terminus.
Database link: O60481 -
Positive control
- ICC/IF: AF22 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. -
Involvement in disease
Heterotaxy, visceral, 1, X-linked
VACTERL association X-linked with or without hydrocephalus
Congenital heart defects, multiple types, 1, X-linked -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Domain
The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation. -
Cellular localization
Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6. - Information by UniProt
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Database links
- Entrez Gene: 7547 Human
- Entrez Gene: 22773 Mouse
- Entrez Gene: 367944 Rat
- Omim: 300265 Human
- SwissProt: O60481 Human
- SwissProt: Q62521 Mouse
- Unigene: 111227 Human
- Unigene: 255890 Mouse
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Alternative names
- Heterotaxy 1 antibody
- HTX antibody
- HTX1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab222124 has been referenced in 3 publications.
- Avsec Ž et al. Base-resolution models of transcription-factor binding reveal soft motif syntax. Nat Genet 53:354-366 (2021). PubMed: 33603233
- Peng T et al. STARR-seq identifies active, chromatin-masked, and dormant enhancers in pluripotent mouse embryonic stem cells. Genome Biol 21:243 (2020). PubMed: 32912294
- Yang SH et al. ZIC3 Controls the Transition from Naive to Primed Pluripotency. Cell Rep 27:3215-3227.e6 (2019). PubMed: 31189106