Key features and details
- Sensitivity: 96 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
Product nameanti-Zika virus IgG ELISA kit
See all IgG kits
Intra-assay Sample n Mean SD CV% 1 24 = 6.7% 2 24 = 2.1% 3 24 = 2.6% Inter-assay Sample n Mean SD CV% 1 12 = 6.2% 2 12 = 5.7% 3 12 = 5.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Other species
The anti-Zika virus IgG capture in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab221844) is designed for the qualitative determination of IgG antibodies to Zika virus in human serum or plasma (citrate, heparin).
Microplates are coated with anti-human IgG-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgG antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color.
Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Zika virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml Cover foil 1 unit IgG Sample Diluent 1 x 100ml HRP Zika Virus Conjugate 1 x 15ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml Zika Virus IgG Cut-off Control 1 x 3ml Zika Virus IgG Microplate 1 x 96 tests Zika Virus IgG Negative Control 1 x 2ml Zika Virus IgG Positive Control 1 x 2ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
ab221844 has not yet been referenced specifically in any publications.