Overview

  • Product name

    anti-Zika virus IgG ELISA kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 24 = 6.7%
    2 24 = 2.1%
    3 24 = 2.6%
    Inter-assay
    Sample n Mean SD CV%
    1 12 = 6.2%
    2 12 = 5.7%
    3 12 = 5.9%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (qualitative)
  • Sensitivity

    96 %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Other species
  • Product overview

    The anti-Zika virus IgG capture in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab221844) is designed for the qualitative determination of IgG antibodies to Zika virus in human serum or plasma (citrate, heparin).


    Microplates are coated with anti-human IgG-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgG antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.


    The intensity of this product is proportional to the amount of specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color.


    Absorbance at 450/620 nm is read using an ELISA microwell plate reader.


     


     

  • Notes

    Zika virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.

     

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 50ml
    Cover foil 1 unit
    IgG Sample Diluent 1 x 100ml
    HRP Zika Virus Conjugate 1 x 15ml
    Stop Solution 1 x 15ml
    TMB Substrate Solution 1 x 15ml
    Zika Virus IgG Cut-off Control 1 x 3ml
    Zika Virus IgG Microplate 1 x 96 tests
    Zika Virus IgG Negative Control 1 x 2ml
    Zika Virus IgG Positive Control 1 x 2ml
  • Research areas

  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab221844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab221844 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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