Product nameanti-Zika virus IgM ELISA kit (μ-capture)
See all IgM kits
Intra-assay Sample n Mean SD CV% 1 24 = 2.7% 2 24 = 1.65% 3 24 = 1.92% Inter-assay Sample n Mean SD CV% 1 12 = 8.02% 2 12 = 5.59% 3 12 = 9.17%
Sample typeSerum, Heparin Plasma, Citrate Plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Other species
Zika virus IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit detects IgM antibodies specific to Zika virus in human serum and plasma. ELISA protocols are available in Spanish and English.
Microplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.
Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Zika virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.
Tested applicationsSuitable for: Indirect ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml Cover foil 1 unit IgM Sample Diluent 1 x 100ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml Zika Virus Conjugate 1 x 15ml Zika Virus IgM Cut-off Control 1 x 3ml Zika Virus IgM Microplate 1 x 96 tests Zika Virus IgM Negative Control 1 x 2ml Zika Virus IgM Positive Control 1 x 2ml
RelevanceIgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
Cellular localizationIsoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
- Constant region of heavy chain of IgM
Our Abpromise guarantee covers the use of ab213327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Gurung S et al. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon. PLoS Pathog 15:e1007507 (2019). Read more (PubMed: 30657788) »
- Gurung S et al. Translational Model of Zika Virus Disease in Baboons. J Virol 92:N/A (2018). Read more (PubMed: 29875247) »