Overview

  • Product name

    anti-Zika virus IgM ELISA kit (μ-capture)
    See all IgM kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 24 = 2.7%
    2 24 = 1.65%
    3 24 = 1.92%
    Inter-assay
    Sample n Mean SD CV%
    1 12 = 8.02%
    2 12 = 5.59%
    3 12 = 9.17%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (qualitative)
  • Sensitivity

    100 %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Other species
  • Product overview

    Zika virus IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit detects IgM antibodies specific to Zika virus in human serum and plasma. ELISA protocols are available in Spanish and English.


    Microplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.


    The intensity of this product is proportional to the amount of specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.


    Absorbance at 450/620 nm is read using an ELISA microwell plate reader.


     


     

  • Notes

    Zika virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.

     

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 50ml
    Cover foil 1 unit
    IgM Sample Diluent 1 x 100ml
    Stop Solution 1 x 15ml
    TMB Substrate Solution 1 x 15ml
    Zika Virus Conjugate 1 x 15ml
    Zika Virus IgM Cut-off Control 1 x 3ml
    Zika Virus IgM Microplate 1 x 96 tests
    Zika Virus IgM Negative Control 1 x 2ml
    Zika Virus IgM Positive Control 1 x 2ml
  • Research areas

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
  • Cellular localization

    Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
  • Alternative names

    • AGM1
    • Constant region of heavy chain of IgM
    • DKFZp686I15196
    • DKFZp686I15212
    • FLJ00385
    • Hepatitis B virus receptor binding protein
    • Ig mu chain C region
    • IGHM
    • IgM heavy chain constant region
    • Immunoglobulin heavy constant mu
    • Immunoglobulin mu chain
    • Imunoglobulin heavy chain
    • Imunoglobulin heavy chain constant region mu
    • Imunoglobulin heavy chain mu constant region
    • MGC104996
    • MGC52291
    • MU
    • VH
    see all

Applications

Our Abpromise guarantee covers the use of ab213327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

See all 4 Publications for this product

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