Key features and details
- Mouse monoclonal [B4] to Zika Virus NS1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Zika virus
- Isotype: IgG1
Product nameAnti-Zika Virus NS1 antibody [B4]
See all Zika Virus NS1 primary antibodies
DescriptionMouse monoclonal [B4] to Zika Virus NS1
Specificityab218546 is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA.
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Zika virus
Recombinant full length protein corresponding to Zika Virus NS1. Produced in HEK-293 cells.
Database link: Q32ZE1
- Recombinant Zika Virus NS1; supernatant from Zika Virus infected Vero cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Constituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab218546 was purified from hybridoma cell culture supernatant by affinity chromatography on Protein G. >95% pure by SDS-PAGE.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab218546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [D11] to Zika Virus NS1 (ab218547).
For coating using 500 ng. ab218546 may be used in combination with clone D11 (ab218547), and has also been shown to effectively pair with itself.
RelevanceZika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.
All lanes : Anti-Zika Virus NS1 antibody [B4] (ab218546) at 1 µg/ml
Lane 1 : Zika virus NS1 at 0.1 µg
Lane 2 : Zika virus NS1 at 0.02 µg
Lane 3 : Zika virus NS1 at 0.004 µg
Lane 4 : Zika virus NS1 at 0.0008 µg
Lane 5 : Dengue virus serotype 1 at 0.1 µg
Lane 6 : Dengue virus serotype 2 at 0.1 µg
Lane 7 : Dengue virus serotype 3 at 0.1 µg
Lane 8 : Dengue virus serotype 4 at 0.1 µg
Lane 9 : West Nile virus NS1 NY99 at 0.1 µg
Lane 10 : Supernatant of Zika virus infected Vero cells at 5 µl
All lanes : Goat anti-Mouse IgG HRP conjugated
4-12% Bis-Tris gel
ab218546 has not yet been referenced specifically in any publications.