Product nameAnti-Zika Virus NS1 antibody [B4]
See all Zika Virus NS1 primary antibodies
DescriptionMouse monoclonal [B4] to Zika Virus NS1
Specificityab218546 is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA.
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Zika virus
Recombinant full length protein corresponding to Zika Virus NS1. Produced in HEK-293 cells.
Database link: Q32ZE1
- Recombinant Zika Virus NS1; supernatant from Zika Virus infected Vero cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab218546 was purified from hybridoma cell culture supernatant by affinity chromatography on Protein G. >95% pure by SDS-PAGE.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab218546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [D11] to Zika Virus NS1 (ab218547).
For coating using 500 ng. ab218546 may be used in combination with clone D11 (ab218547), and has also been shown to effectively pair with itself.
RelevanceZika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.
All lanes : Anti-Zika Virus NS1 antibody [B4] (ab218546) at 1 µg/ml
Lane 1 : Zika virus NS1 at 0.1 µg
Lane 2 : Zika virus NS1 at 0.02 µg
Lane 3 : Zika virus NS1 at 0.004 µg
Lane 4 : Zika virus NS1 at 0.0008 µg
Lane 5 : Dengue virus serotype 1 at 0.1 µg
Lane 6 : Dengue virus serotype 2 at 0.1 µg
Lane 7 : Dengue virus serotype 3 at 0.1 µg
Lane 8 : Dengue virus serotype 4 at 0.1 µg
Lane 9 : West Nile virus NS1 NY99 at 0.1 µg
Lane 10 : Supernatant of Zika virus infected Vero cells at 5 µl
All lanes : Goat anti-Mouse IgG HRP conjugated
4-12% Bis-Tris gel
ab218546 has not yet been referenced specifically in any publications.