Anti-Zika Virus NS1 antibody [B4] (ab218546)

Overview

  • Product name

    Anti-Zika Virus NS1 antibody [B4]
    See all Zika Virus NS1 primary antibodies
  • Description

    Mouse monoclonal [B4] to Zika Virus NS1
  • Host species

    Mouse
  • Specificity

    ab218546 is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA.
  • Tested applications

    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Zika virus
  • Immunogen

    Recombinant full length protein corresponding to Zika Virus NS1. Produced in HEK-293 cells.
    Database link: Q32ZE1

  • Positive control

    • Recombinant Zika Virus NS1; supernatant from Zika Virus infected Vero cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab218546 was purified from hybridoma cell culture supernatant by affinity chromatography on Protein G. >95% pure by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    B4
  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab218546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [D11] to Zika Virus NS1 (ab218547).

For coating using 500 ng. ab218546 may be used in combination with clone D11 (ab218547), and has also been shown to effectively pair with itself.

Target

  • Relevance

    Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.
  • Database links

    Images

    • All lanes : Anti-Zika Virus NS1 antibody [B4] (ab218546) at 1 µg/ml

      Lane 1 : Zika virus NS1 at 0.1 µg
      Lane 2 : Zika virus NS1 at 0.02 µg
      Lane 3 : Zika virus NS1 at 0.004 µg
      Lane 4 : Zika virus NS1 at 0.0008 µg
      Lane 5 : Dengue virus serotype 1 at 0.1 µg
      Lane 6 : Dengue virus serotype 2 at 0.1 µg
      Lane 7 : Dengue virus serotype 3 at 0.1 µg
      Lane 8 : Dengue virus serotype 4 at 0.1 µg
      Lane 9 : West Nile virus NS1 NY99 at 0.1 µg
      Lane 10 : Supernatant of Zika virus infected Vero cells at 5 µl

      Secondary
      All lanes : Goat anti-Mouse IgG HRP conjugated


      4-12% Bis-Tris gel

    References

    ab218546 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab218546.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up