Anti-Zika Virus NS1 antibody [F9] - BSA and Azide free (ab218548)

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Key features and details

  • Mouse monoclonal [F9] to Zika Virus NS1 - BSA and Azide free
  • Suitable for: ELISA
  • Reacts with: Zika virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-Zika Virus NS1 antibody [F9] - BSA and Azide free
    See all Zika Virus NS1 primary antibodies

  • Description

    Mouse monoclonal [F9] to Zika Virus NS1 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    This antibody is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus, West Nile Virus and Yellow Fever Virus

  • Tested applications

    Suitable for: ELISAmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Zika virus

  • Immunogen

    Recombinant full length protein corresponding to Zika virus Zika Virus NS1 aa 791-1142. Full length recombinant NS1 (Non Structural protain 1) protein of Zika virus produced in HEK293 cells.
    Sequence:

    DVGCSVDFSKKETRCGTGVFIYNDVEAWRDRYKYHPDSPRRLAAAVKQAW EEGICGISSV SRMENIMWKSVEGELNAILEENGVQLTVVVGSVKNPMW RGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLE HRAWNSFLVEDHGFGVFHTSVWLKVREDYSLECD PAVIGTAVKGREAA HSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGVEESD LI IPKSLAGPLSHHNTREGYRTQVKGPWHSEELEIRFEECPGTKVYVEETCG TRGPSLRS TTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKE PESNLVRSMVTA


    Database link: Q32ZE1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Direct ELISA - Zika virus NS1 (Uganda and Suriname strains)

  • General notes

    For long term storage at +4°C the addition of 0.09% w/v sodium azide is recommended

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Constituent: 100% PBS

    Preservative: None present. 0.2 µm filtered. For long term storage at +4°C the addition of 0.09% w/v sodium azide is recommended

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from hybridoma cell culture supernatant. >95% pure by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    F9

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab218548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml.

For Direct ELISA, the plate was coated with 100ng of antigen per well, then blocked with 2% BSA and the detection antibody used was Goat anti-mouse IgG HRP conjugated at 1/2000.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.

    • Database links

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab218548? Please let us know so that we can cite the reference in this datasheet.

      ab218548 has not yet been referenced specifically in any publications.

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