Anti-Zika Virus NS1 antibody [F9] - BSA and Azide free (ab218548)

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Overview

  • Product name

    Anti-Zika Virus NS1 antibody [F9] - BSA and Azide free
    See all Zika Virus NS1 primary antibodies

  • Description

    Mouse monoclonal [F9] to Zika Virus NS1 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    This antibody is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus, West Nile Virus and Yellow Fever Virus

  • Tested applications

    Suitable for: ELISAmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Zika virus

  • Immunogen

    Recombinant full length protein corresponding to Zika virus Zika Virus NS1 aa 791-1142. Full length recombinant NS1 (Non Structural protain 1) protein of Zika virus produced in HEK293 cells.
    Sequence:

    DVGCSVDFSKKETRCGTGVFIYNDVEAWRDRYKYHPDSPRRLAAAVKQAW EEGICGISSV SRMENIMWKSVEGELNAILEENGVQLTVVVGSVKNPMW RGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLE HRAWNSFLVEDHGFGVFHTSVWLKVREDYSLECD PAVIGTAVKGREAA HSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGVEESD LI IPKSLAGPLSHHNTREGYRTQVKGPWHSEELEIRFEECPGTKVYVEETCG TRGPSLRS TTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKE PESNLVRSMVTA


    Database link: Q32ZE1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Direct ELISA - Zika virus NS1 (Uganda and Suriname strains)

  • General notes

    For long term storage at +4°C the addition of 0.09% w/v sodium azide is recommended

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.4
    Constituent: 100% PBS

    Preservative: None present. 0.2 µm filtered. For long term storage at +4°C the addition of 0.09% w/v sodium azide is recommended
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from hybridoma cell culture supernatant. >95% pure by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    F9

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab218548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml.

For Direct ELISA, the plate was coated with 100ng of antigen per well, then blocked with 2% BSA and the detection antibody used was Goat anti-mouse IgG HRP conjugated at 1/2000.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.

    • Database links

      References

      ab218548 has not yet been referenced specifically in any publications.

      Publishing research using ab218548? Please let us know so that we can cite the reference in this datasheet.

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