Overview

  • Product name

    Anti-Zinc Alpha 2 Glycoprotein antibody
    See all Zinc Alpha 2 Glycoprotein primary antibodies
  • Description

    Rabbit polyclonal to Zinc Alpha 2 Glycoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Zinc Alpha 2 Glycoprotein aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab133780)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total Protein; Human Heart; Human Lung; Human Adipose. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: HeLa.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
  • Tissue specificity

    Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha 2 glycoprotein 1 zinc antibody
    • Alpha 2 glycoprotein 1 zinc binding antibody
    • Alpha 2 glycoprotein zinc antibody
    • Azgp1 antibody
    • ZA2G antibody
    • ZA2G_HUMAN antibody
    • ZAG antibody
    • Zinc alpha 2 glycoprotein precursor antibody
    • Zinc-alpha-2-glycoprotein antibody
    • Zn alpha 2 glycoprotein antibody
    • Zn alpha 2 GP antibody
    • Zn alpha2 glycoprotein antibody
    • Zn-alpha-2-glycoprotein antibody
    • Zn-alpha-2-GP antibody
    • ZNGP1 antibody
    see all

Images

  • All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    Zinc Alpha 2 Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab117275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117275 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab117275 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Liu J  et al. AZGP1 inhibits soft tissue sarcoma cells invasion and migration. BMC Cancer 18:89 (2018). WB, IHC-P ; Human . Read more (PubMed: 29357838) »
  • Gao SX  et al. ZAG alleviates HFD-induced insulin resistance accompanied with decreased lipid depot in skeletal muscle in mice. J Lipid Res 59:2277-2286 (2018). Read more (PubMed: 30318474) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

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Verified customer

Submitted Jun 20 2018

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