Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Product nameAnti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]
See all Zinc Alpha 2 Glycoprotein primary antibodies
DescriptionRabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Zinc Alpha 2 Glycoprotein aa 50-150. The exact sequence is proprietary.
Database link: P25311
- Human Plasma and HepG2 cell lines; Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionStimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
Tissue specificityBlood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.
Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
- Information by UniProt
- Alpha 2 glycoprotein 1 zinc antibody
- Alpha 2 glycoprotein 1 zinc binding antibody
- Alpha 2 glycoprotein zinc antibody
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg with NFDM/TBST at 5 %
Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 at 10 µg with NFDM/TBST at 5 %
Goat Anti-rabbit HRP at 1/1000 dilution
Predicted band size: 34 kDa
Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution
Lane 1 : Human plasma with NFDM/TBST
Lane 2 : Negative control with NFDM/TBST
Blocking peptides at 5 % per lane.
All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution
Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab180574 has been referenced in 1 publication.
- Xue Y et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). WB ; Human . PubMed: 25561225