Anti-Zinc finger antiviral protein antibody (ab154680)

Overview

  • Product name
    Anti-Zinc finger antiviral protein antibody
    See all Zinc finger antiviral protein primary antibodies
  • Description
    Rabbit polyclonal to Zinc finger antiviral protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Zinc finger antiviral protein aa 401-721 (internal sequence).
    Database link: Q7Z2W4

  • Positive control
    • A431 whole cell lysate; A431 cells; Human hepatoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 101 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Induces an innate immunity to viral infections by preventing the accumulation of viral RNAs in the cytoplasm. Seems to recruit the RNA processing exosome to degrade the target RNAs. Inhibits alphavirus and filovirus replication.
  • Sequence similarities
    Contains 4 C3H1-type zinc fingers.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • Domain
    The second and fourth zinc fingers are involved in binding to specific viral RNAs.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase diphtheria toxin like 13 antibody
    • ARTD13 antibody
    • FLB6421 antibody
    • PARP13 antibody
    • ZAP antibody
    • ZC3H2 antibody
    • ZC3HAV 1 antibody
    • Zc3hav1 antibody
    • ZC3HDC 2 antibody
    • ZC3HDC2 antibody
    • ZCCHV antibody
    • ZCCHV_HUMAN antibody
    • Zinc finger antiviral protein antibody
    • Zinc finger CCCH domain containing protein 2 antibody
    • Zinc finger CCCH domain-containing antiviral protein 1 antibody
    • Zinc finger CCCH domain-containing protein 2 antibody
    • Zinc finger CCCH type antiviral 1 antibody
    • Zinc finger CCCH type antiviral protein 1 antibody
    • Zinc finger CCCH-type antiviral protein 1 antibody
    see all

Images

  • Anti-Zinc finger antiviral protein antibody (ab154680) at 1/10000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 101 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Zinc finger antiviral protein with ab154680 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling Zinc finger antiviral protein with ab154680 at 1/500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells induced to neurons with)
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 12.5%)
Loading amount
20 µg
Specification
Mouse embryonic stem cells induced to neurons with
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Olivier Martel Paradis

Verified customer

Submitted Mar 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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