Key features and details
- Rabbit polyclonal to Zinc finger antiviral protein
- Suitable for: ELISA, ICC/IF, IHC-P
- Isotype: IgG
Product nameAnti-Zinc finger antiviral protein antibody
See all Zinc finger antiviral protein primary antibodies
Predicted to work with: Human, Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human Zinc finger antiviral protein aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
|ELISA||Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab73368).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInduces an innate immunity to viral infections by preventing the accumulation of viral RNAs in the cytoplasm. Seems to recruit the RNA processing exosome to degrade the target RNAs. Inhibits alphavirus and filovirus replication.
Sequence similaritiesContains 4 C3H1-type zinc fingers.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
DomainThe second and fourth zinc fingers are involved in binding to specific viral RNAs.
Cellular localizationCytoplasm. Nucleus. Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner.
- Information by UniProt
- ADP ribosyltransferase diphtheria toxin like 13 antibody
- ARTD13 antibody
- FLB6421 antibody
ICC/IF image of ab73369 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab73369, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100$% methanol fixed (5 mins) HePG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
IHC image of Zinc finger antiviral protein staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73369, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab73369 has been referenced in 3 publications.
- Liu CH et al. Battle between influenza A virus and a newly identified antiviral activity of the PARP-containing ZAPL protein. Proc Natl Acad Sci U S A 112:14048-53 (2015). PubMed: 26504237
- Gläsker S et al. The alternate triad motif of the poly(ADP-ribose) polymerase-like domain of the human zinc finger antiviral protein is essential for its antiviral activity. J Gen Virol 95:816-22 (2014). WB ; Human . PubMed: 24457973
- Hayakawa S et al. ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses. Nat Immunol 12:37-44 (2011). WB, IP . PubMed: 21102435