Zinc Quantification Kit (Fluorometric) (ab176725)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 13 ng/ml
Overview
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Product name
Zinc Quantification Kit (Fluorometric)
See all Zinc kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
>= 13 ng/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).
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Notes
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 15ml Zn Detector 1 vial ZnCl2 Standard 1 vial -
Research areas
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Relevance
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. -
Alternative names
- Zn
- Zn++
- Zn2+
Images
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ZnCl2 dose response curve
ZnCl2 dose response was measured on a 96-well black plate with Zinc Quantification Kit (Fluorometric) (ab176725). As low as ~ 0.2 µM Zn2+ can be detected with 5 minutes incubation time (n=3).
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab176725 has been referenced in 4 publications.
- Kim JM et al. Impaired Cu-Zn Superoxide Dismutase (SOD1) and Calcineurin (Cn) Interaction in ALS: A Presumed Consequence for TDP-43 and Zinc Aggregation in Tg SOD1G93A Rodent Spinal Cord Tissue. Neurochem Res 44:228-233 (2019). PubMed: 29299811
- Guillory RJ et al. Preclinical In-Vivo Evaluation and Screening of Zinc Based Degradable Metals for Endovascular Stents. JOM (1989) 71:1436-1446 (2019). PubMed: 33731979
- Reinhardt S et al. Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks. Sci Rep 8:1329 (2018). Mouse . PubMed: 29358714
- Li C et al. PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism. Dev Cell 46:441-455.e8 (2018). PubMed: 30100261