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Zinc Quantification Kit (Fluorometric) (ab176725)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (3)

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ZnCl2 dose response curve

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
    • Sensitivity: 13 ng/ml

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    Overview

    • Product name

      Zinc Quantification Kit (Fluorometric)
      See all Zinc kits
    • Detection method

      Fluorescent
    • Sample type

      Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
    • Assay type

      Quantitative
    • Sensitivity

      >= 13 ng/ml
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).

    • Notes

      Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 200 tests
      Assay Buffer 1 x 15ml
      Zn Detector 1 vial
      ZnCl2 Standard 1 vial
    • Research areas

      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cell Biology
      • Other Antibodies
      • Drugs and Chemicals
      • Kits/ Lysates/ Other
      • Kits
      • Cell Signaling Kits
      • Ions and Metal Assay Kits
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Ion and metal assay kits
    • Relevance

      Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function.
    • Alternative names

      • Zn
      • Zn++
      • Zn2+

    Associated products

      Images

      • ZnCl2 dose response curve
        ZnCl2 dose response curve

        ZnCl2 dose response was measured on a 96-well black plate with Zinc Quantification Kit (Fluorometric) (ab176725). As low as ~ 0.2 µM Zn2+ can be detected with 5 minutes incubation time (n=3).

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab176725? Please let us know so that we can cite the reference in this datasheet.

      ab176725 has been referenced in 3 publications.

      • Kim JM  et al. Impaired Cu-Zn Superoxide Dismutase (SOD1) and Calcineurin (Cn) Interaction in ALS: A Presumed Consequence for TDP-43 and Zinc Aggregation in Tg SOD1G93A Rodent Spinal Cord Tissue. Neurochem Res 44:228-233 (2019). PubMed: 29299811
      • Li C  et al. PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism. Dev Cell 46:441-455.e8 (2018). PubMed: 30100261
      • Reinhardt S  et al. Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks. Sci Rep 8:1329 (2018). Mouse . PubMed: 29358714

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I cannot seem to find the volumes provided in the tubes of the separate components: the Zn Detector solution and the ZnCl2 Standard solution. Could you please provide me with volumes of each of these vials?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 16 2015

      Answer

      Thank you very much for your kind feedback.

      I can confirm you that The Zn Detection solution vial contains 50 ul and the ZnCl2 standard vial contains 100 ul.

      Do not hesitate to contact us if you need further assistance.

      Read More

      Abcam Scientific Support

      Answered on Jun 16 2015

      Question

      How reproducible is this kit? What is the inter-assay CV, for example?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 10 2014

      Answer

      The reproducibility of the product is very good. In lab testing, the average interassay CV was ~5% and often is as low as 2%.

      Read More

      Caitlin Valued Customer

      Abcam Scientific Support

      Answered on Jun 10 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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