Zinc Quantification Kit (Fluorometric) (ab176725)

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Overview

  • Product name

    Zinc Quantification Kit (Fluorometric)
    See all Zinc kits

  • Detection method

    Fluorescent

  • Sample type

    Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts

  • Assay type

    Quantitative

  • Sensitivity

    >= 13 ng/ml

  • Species reactivity

    Reacts with: Other species, Mammals

  • Product overview

    Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).

  • Notes

    Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.

  • Platform

    Microplate reader

Properties

Images

  • ZnCl2 dose response curve
    ZnCl2 dose response curve

    ZnCl2 dose response was measured on a 96-well black plate with Zinc Quantification Kit (Fluorometric) (ab176725). As low as ~ 0.2 µM Zn2+ can be detected with 5 minutes incubation time (n=3).

Protocols

References

This product has been referenced in:

  • Kim JM  et al. Impaired Cu-Zn Superoxide Dismutase (SOD1) and Calcineurin (Cn) Interaction in ALS: A Presumed Consequence for TDP-43 and Zinc Aggregation in Tg SOD1G93A Rodent Spinal Cord Tissue. Neurochem Res 44:228-233 (2019). Read more (PubMed: 29299811) »
  • Reinhardt S  et al. Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks. Sci Rep 8:1329 (2018). Mouse . Read more (PubMed: 29358714) »
See all 2 Publications for this product

Publishing research using ab176725? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I cannot seem to find the volumes provided in the tubes of the separate components: the Zn Detector solution and the ZnCl2 Standard solution. Could you please provide me with volumes of each of these vials?

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Answer

Thank you very much for your kind feedback.

I can confirm you that The Zn Detection solution vial contains 50 ul and the ZnCl2 standard vial contains 100 ul.

Do not hesitate to contact us if you need further assistance.

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Question

How reproducible is this kit? What is the inter-assay CV, for example?

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Answer

The reproducibility of the product is very good. In lab testing, the average interassay CV was ~5% and often is as low as 2%.

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