Overview

  • Product name

    Anti-ZIP-12 antibody
  • Description

    Rabbit polyclonal to ZIP-12
  • Host species

    Rabbit
  • Specificity

    At least four isoforms of ZIP-12 are known to exist. ab106570 is predicted not to cross-react with other SLC39 family members.
  • Tested applications

    Suitable for: ICC/IF, WB, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ZIP-12 (N terminal).
    Database link: NP_001138667

  • Positive control

    • HepG2 cell lysate
  • General notes

    Previously labelled as SLC39A12. 

Properties

Applications

Our Abpromise guarantee covers the use of ab106570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77, 73, 46 kDa.
ICC Use a concentration of 2.5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Acts as a zinc-influx transporter (Potential). May be partly involved in the outbreak of schizophrenia.
  • Tissue specificity

    Expressed in brain and eye.
  • Sequence similarities

    Belongs to the ZIP transporter (TC 2.A.5) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA570F3.1 antibody
    • FLJ30499 antibody
    • LIV 1 subfamily of ZIP zinc transporter 8 antibody
    • LIV-1 subfamily of ZIP zinc transporter 8 antibody
    • LZT Hs8 antibody
    • LZT-Hs8 antibody
    • LZTHs8 antibody
    • MGC43205 antibody
    • MGC51099 antibody
    • S39AC_HUMAN antibody
    • SLC39A12 antibody
    • Solute carrier family 39 (metal ion transporter) member 12 antibody
    • Solute carrier family 39 (zinc transporter) member 12 antibody
    • Solute carrier family 39 member 12 antibody
    • Zinc transporter ZIP12 antibody
    • ZIP 12 antibody
    • ZIP-12 antibody
    • ZIP12 antibody
    • Zrt and Irt like protein 12 antibody
    • Zrt- and Irt-like protein 12 antibody
    see all

Images

  • Lane 1 : Anti-ZIP-12 antibody (ab106570) at 0.5 µg/ml
    Lane 2 : Anti-ZIP-12 antibody (ab106570) at 1 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 77, 73, 46 kDa

  • Immunofluorescence of ZIP-12 in mouse liver tissue with ab106570 at 20 ug/mL.

  • Immunohistochemistry of ZIP-12 in mouse liver tissue with ab106570 at 2.5 µg/ml.

References

This product has been referenced in:

  • Klingeborn M  et al. Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers. Sci Rep 7:4901 (2017). WB ; Human, Pig . Read more (PubMed: 28687758) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Pig Cell lysate - whole cell (Retinal Pigmented Epithelium (RPE) cells)
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris)
Loading amount
20 µg
Specification
Retinal Pigmented Epithelium (RPE) cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Mikael Klingeborn

Verified customer

Submitted May 23 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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