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  1. Link

    zip-4-antibody-ab229579.pdf

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Anti-ZIP-4 antibody (ab229579)

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Western blot - Anti-ZIP-4 antibody (ab229579)

    Key features and details

    • Rabbit polyclonal to ZIP-4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-ZIP-4 antibody
      See all ZIP-4 primary antibodies
    • Description

      Rabbit polyclonal to ZIP-4
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human ZIP-4 (internal sequence). The exact sequence is proprietary.
      Database link: Q6P5W5

    • Positive control

      • WB: hZIP-4-transfected HEK-293T cells.
    • General notes

      Protein previously labeled as SLC39A4.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Endosome
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Vitamins / minerals

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HEK293 whole cell lysate (ab7902)

    Applications

    Our Abpromise guarantee covers the use of ab229579 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.

    Target

    • Function

      Plays an important role in cellular zinc homeostasis as a zinc transporter. Regulated in response to zinc availability.
    • Tissue specificity

      Highly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum.
    • Involvement in disease

      Acrodermatitis enteropathica, zinc-deficiency type
    • Sequence similarities

      Belongs to the ZIP transporter (TC 2.A.5) family.
    • Cellular localization

      Cell membrane. Recycling endosome membrane. Colocalized with TFRC in the recycling endosomes. Cycles between endosomal compartments and the plasma membrane in response to zinc availability.
    • Target information above from: UniProt accession Q6P5W5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55630 Human
      • Omim: 607059 Human
      • SwissProt: Q6P5W5 Human
      • Unigene: 521934 Human
      • Alternative names

        • 1600025H15Rik antibody
        • Acrodermatitis enteropathica zinc deficiency type antibody
        • Activated in W/Wv mouse stomach 2 antibody
        • AEZ antibody
        • AU041686 antibody
        • AWMS2 antibody
        • FLJ20327 antibody
        • MGC156705 antibody
        • MGC74741 antibody
        • S39A4_HUMAN antibody
        • Slc39a4 antibody
        • Solute carrier family 39 member 4 antibody
        • Ssolute carrier family 39 zinc transporter member 4 antibody
        • Zinc transporter ZIP4 antibody
        • ZIP-4 antibody
        • ZIP4 antibody
        • Zrt and Irt like protein 4 antibody
        • Zrt- and Irt-like protein 4 antibody
        see all

      Images

      • Western blot - Anti-ZIP-4 antibody (ab229579)
        Western blot - Anti-ZIP-4 antibody (ab229579)
        All lanes : Anti-ZIP-4 antibody (ab229579) at 1/4000 dilution

        Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
        Lane 2 : hZIP-4-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 68 kDa



        7.5% SDS-PAGE gel.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab229579? Please let us know so that we can cite the reference in this datasheet.

      ab229579 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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