Anti-ZIP Kinase antibody (ab2057)
Key features and details
- Rabbit polyclonal to ZIP Kinase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZIP Kinase antibody
See all ZIP Kinase primary antibodies -
Description
Rabbit polyclonal to ZIP Kinase -
Host species
Rabbit -
Specificity
It has no cross reactivity to DAP kinase. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ZIP Kinase aa 250-350 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Database link: O43293 -
Positive control
- HeLa whole cell lysate or Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. |
Target
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Function
Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1. -
Cellular localization
Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase. - Information by UniProt
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Database links
- Entrez Gene: 1613 Human
- Omim: 603289 Human
- SwissProt: O43293 Human
- Unigene: 631844 Human
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Alternative names
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence of Jurkat cells staining ZIP Kinase with ab2057 at 10µg/ml
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Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.
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All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
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Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab2057 has been referenced in 3 publications.
- Yuan S et al. Tumor-associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18:1840-1846 (2019). PubMed: 31423252
- Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
- Oster B et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). PubMed: 18089732