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Synthetic peptide corresponding to Human ZIP Kinase aa 400 to the C-terminus (C terminal).
Database link: O43293
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab51602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab51602 was shown to specifically react with ZIP Kinase in wild-type HAP1 cells. No band was observed when ZIP Kinase knockout samples were examined. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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