Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1636Y] to ZIP Kinase
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Product nameAnti-ZIP Kinase antibody [EPR1636Y]
See all ZIP Kinase primary antibodies
DescriptionRabbit monoclonal [EPR1636Y] to ZIP Kinase
Specificityab51602 recognizes ZIP Kinase.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ZIP Kinase aa 400 to the C-terminus (C terminal).
Database link: O43293
- HeLa cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab51602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSerine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
modificationsUbiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1.
Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
- Information by UniProt
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab51602 was shown to specifically react with ZIP Kinase in wild-type HAP1 cells. No band was observed when ZIP Kinase knockout samples were examined. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution + HeLa cell lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51602 has been referenced in 3 publications.
- Plumbly W et al. L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks. Sci Rep 9:13810 (2019). PubMed: 31554851
- Kocher BA et al. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res 13:358-67 (2015). WB ; Human . PubMed: 25304685
- Marina O et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res 70:1344-55 (2010). WB ; Human . PubMed: 20124481