Overview

  • Product name

  • Description

    Rabbit polyclonal to ZKSCAN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ZKSCAN3 aa 8-275.
    Database link: Q9BRR0

  • Positive control

    • WB: Mouse heart, spleen and lung lysates. IHC-P: Human lymph node tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 44,61 kDa (predicted molecular weight: 44,61 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression.
  • Tissue specificity

    Expressed in colorectal cancers (at protein level).
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
    Contains 1 SCAN box domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • dJ874C20.1 antibody
    • mszf35 antibody
    • SCAN-KRAB-zinc finger protein antibody
    • Skz1 antibody
    • Zf47 antibody
    • Zfp-47 antibody
    • ZFP306 antibody
    • Zfp307 antibody
    • Zfp47 antibody
    • Zinc finger and SCAN domain containing protein 13 antibody
    • Zinc finger and SCAN domain-containing protein 13 antibody
    • Zinc finger protein 306 antibody
    • Zinc finger protein 306, isoform CRA_a antibody
    • Zinc finger protein 307 antibody
    • Zinc finger protein 309 antibody
    • Zinc finger protein 47 homolog antibody
    • Zinc finger protein with KRAB and SCAN domains 3 antibody
    • Zinc finger protein with KRAB and SCAN domains 3 antibody
    • Zinc finger protein zfp47 antibody
    • Zinc finger with KRAB and SCAN domains 3 antibody
    • ZKSC3_HUMAN antibody
    • ZKSCAN3 antibody
    • ZNF309 antibody
    • ZSCAN13 antibody
    • ZSCAN35 antibody
    see all

Images

  • All lanes : Anti-ZKSCAN3 antibody (ab223477) at 1/500 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse lung lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 44,61 kDa
    Observed band size: 44,61 kDa
    why is the actual band size different from the predicted?

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZKSCAN3 (green) using ab223477 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human lymph node tissue stained for ZKSCAN3 using ab223477 at 1/100 dilution in immunohistochemical analysis.

References

ab223477 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223477.
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