Key features and details
- Rabbit polyclonal to ZKSCAN3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZKSCAN3 antibody
See all ZKSCAN3 primary antibodies
DescriptionRabbit polyclonal to ZKSCAN3
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ZKSCAN3 aa 8-275.
Database link: Q9BRR0
- WB: Mouse heart, spleen and lung lysates. IHC-P: Human lymph node tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 44,61 kDa (predicted molecular weight: 44,61 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionActs as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression.
Tissue specificityExpressed in colorectal cancers (at protein level).
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain.
- Information by UniProt
- dJ874C20.1 antibody
- mszf35 antibody
- SCAN-KRAB-zinc finger protein antibody
All lanes : Anti-ZKSCAN3 antibody (ab223477) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse lung lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44,61 kDa
Observed band size: 44,61 kDa why is the actual band size different from the predicted?
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZKSCAN3 (green) using ab223477 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human lymph node tissue stained for ZKSCAN3 using ab223477 at 1/100 dilution in immunohistochemical analysis.
ab223477 has not yet been referenced specifically in any publications.