Anti-ZNDR1 antibody (ab254992)
Key features and details
- Rabbit polyclonal to ZNDR1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNDR1 antibody -
Description
Rabbit polyclonal to ZNDR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Recombinant fragment corresponding to Human ZNDR1 aa 50 to the C-terminus.
Database link: Q9P1U0 -
Positive control
- IHC-P: Human fallopian tube tissue. ICC/IF: HEK-293 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| All applications |
Cow
Chimpanzee
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. -
Sequence similarities
Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Contains 1 TFIIS-type zinc finger. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 471952 Chimpanzee
- Entrez Gene: 535288 Cow
- Entrez Gene: 30834 Human
- Omim: 607525 Human
- SwissProt: Q1XHV8 Chimpanzee
- SwissProt: Q1RMP0 Cow
- SwissProt: Q9P1U0 Human
- Unigene: 57813 Human
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Alternative names
- DNA directed RNA polymerase I subunit RPA12 antibody
- DNA-directed RNA polymerase I subunit RPA12 antibody
- HTEX 6 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNDR1 antibody (ab254992)Paraffin-embedded human fallopian tube tissue stained for ZNDR1 using ab254992 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZNDR1 antibody (ab254992)PFA fixed, Triton X-100 permeabilized HEK293 (Human epithelial cell line from embryonic kidney) cells labeling ZNDR1 using ab254992 at 4µg/ml (green) in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab254992 has not yet been referenced specifically in any publications.
