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    zndr1-antibody-ab254992.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-ZNDR1 antibody (ab254992)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNDR1 antibody (ab254992)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZNDR1 antibody (ab254992)

Key features and details

  • Rabbit polyclonal to ZNDR1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ZNDR1 protein (ab176045)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ZNDR1 antibody
  • Description

    Rabbit polyclonal to ZNDR1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human ZNDR1 aa 50 to the C-terminus.
    Database link: Q9P1U0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human fallopian tube tissue. ICC/IF: HEK-293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol I Transcription
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human ZNDR1 protein (ab176045)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab254992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
All applications
Cow
Chimpanzee
Application Abreviews Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors.
  • Sequence similarities

    Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localization

    Nucleus > nucleolus.
  • Target information above from: UniProt accession Q9P1U0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 471952 Chimpanzee
    • Entrez Gene: 535288 Cow
    • Entrez Gene: 30834 Human
    • Omim: 607525 Human
    • SwissProt: Q1XHV8 Chimpanzee
    • SwissProt: Q1RMP0 Cow
    • SwissProt: Q9P1U0 Human
    • Unigene: 57813 Human
    • Alternative names

      • DNA directed RNA polymerase I subunit RPA12 antibody
      • DNA-directed RNA polymerase I subunit RPA12 antibody
      • HTEX 6 antibody
      • hZR14 antibody
      • RNA polymerase I small specific subunit Rpa12 antibody
      • RPA12 antibody
      • RPA12_HUMAN antibody
      • tctex 6 antibody
      • TCTEX6 antibody
      • TEX6 antibody
      • Transcription associated zinc ribbon protein antibody
      • Zinc ribbon domain containing 1 antibody
      • Zinc ribbon domain containing protein 1 antibody
      • Zinc ribbon domain-containing protein 1 antibody
      • ZNRD1 antibody
      • ZR14 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNDR1 antibody (ab254992)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNDR1 antibody (ab254992)
      Paraffin-embedded human fallopian tube tissue stained for ZNDR1 using ab254992 at 1/1000 dilution in immunohistochemical analysis.
    • Immunocytochemistry/ Immunofluorescence - Anti-ZNDR1 antibody (ab254992)
      Immunocytochemistry/ Immunofluorescence - Anti-ZNDR1 antibody (ab254992)
      PFA fixed, Triton X-100 permeabilized HEK293 (Human epithelial cell line from embryonic kidney) cells labeling ZNDR1 using ab254992 at 4µg/ml (green) in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab254992? Please let us know so that we can cite the reference in this datasheet.

    ab254992 has not yet been referenced specifically in any publications.

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