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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-ZNF136 antibody [EPR8225] (ab129100)

  • Datasheet
  • SDS
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Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
  • OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
  • Anti-ZNF136 antibody [EPR8225] (ab129100)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8225] to ZNF136
  • Suitable for: WB
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ZNF136 antibody [EPR8225]
    See all ZNF136 primary antibodies
  • Description

    Rabbit monoclonal [EPR8225] to ZNF136
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ZNF136 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal liver and 293T cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8225
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Alternative Versions

    • Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.
    • Tissue specificity

      Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 14 C2H2-type zinc fingers.
      Contains 1 KRAB domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P52737 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7695 Human
      • Omim: 604078 Human
      • SwissProt: P52737 Human
      • Unigene: 479874 Human
      • Alternative names

        • pHZ 20 antibody
        • Zinc finger protein 136 antibody
        • Zinc finger protein 136 clone pHZ 20 antibody
        • ZN136_HUMAN antibody
        • ZNF136 antibody
        see all

      Images

      • Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Lanes 1-3 : Anti-ZNF136 antibody [EPR8225] (ab129100) at 1/1000 dilution
        Lane 4 : Anti-ZNF136 antibody [EPR8225] (ab129100)

        Lane 1 : Human fetal heart cell lysates at 10 µg
        Lane 2 : Human fetal liver cell lysates at 10 µg
        Lane 3 : 293T cell lysates at 10 µg
        Lane 4 : HeLa cell lysates

        Secondary
        Lanes 1-3 : Goat anti-Rabbit HRP at 1/2000 dilution


        Predicted band size: 63 kDa

      • OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
        OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ZNF136 antibody [EPR8225] (ab129100)
        Anti-ZNF136 antibody [EPR8225] (ab129100)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab129100? Please let us know so that we can cite the reference in this datasheet.

      ab129100 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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