Recombinant Anti-ZNF136 antibody [EPR8225] (ab129100)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8225] to ZNF136
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-ZNF136 antibody [EPR8225]
See all ZNF136 primary antibodies -
Description
Rabbit monoclonal [EPR8225] to ZNF136 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ZNF136 aa 50-150. The exact sequence is proprietary.
-
Positive control
- Human fetal heart, fetal liver and 293T cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8225 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
Target
-
Function
May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain. -
Tissue specificity
Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 14 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7695 Human
- Omim: 604078 Human
- SwissProt: P52737 Human
- Unigene: 479874 Human
-
Alternative names
- pHZ 20 antibody
- Zinc finger protein 136 antibody
- Zinc finger protein 136 clone pHZ 20 antibody
see all
Images
-
Lanes 1-3 : Anti-ZNF136 antibody [EPR8225] (ab129100) at 1/1000 dilution
Lane 4 : Anti-ZNF136 antibody [EPR8225] (ab129100)
Lane 1 : Human fetal heart cell lysates at 10 µg
Lane 2 : Human fetal liver cell lysates at 10 µg
Lane 3 : 293T cell lysates at 10 µg
Lane 4 : HeLa cell lysates
Secondary
Lanes 1-3 : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab129100 has not yet been referenced specifically in any publications.