Recombinant
RabMAb

Recombinant Anti-ZNF136 antibody [EPR8225] (ab129100)

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Overview

  • Product name

    Anti-ZNF136 antibody [EPR8225]
    See all ZNF136 primary antibodies

  • Description

    Rabbit monoclonal [EPR8225] to ZNF136

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ZNF136 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal liver and 293T cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab129100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.

    • Tissue specificity

      Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 14 C2H2-type zinc fingers.
      Contains 1 KRAB domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • pHZ 20 antibody
        • Zinc finger protein 136 antibody
        • Zinc finger protein 136 clone pHZ 20 antibody
        • ZN136_HUMAN antibody
        • ZNF136 antibody
        see all

      Images

      • Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Lanes 1-3 : Anti-ZNF136 antibody [EPR8225] (ab129100) at 1/1000 dilution
        Lane 4 : Anti-ZNF136 antibody [EPR8225] (ab129100)

        Lane 1 : Human fetal heart cell lysates at 10 µg
        Lane 2 : Human fetal liver cell lysates at 10 µg
        Lane 3 : 293T cell lysates at 10 µg
        Lane 4 : HeLa cell lysates

        Secondary
        Lanes 1-3 : Goat anti-Rabbit HRP at 1/2000 dilution


        Predicted band size: 63 kDa

      • Other - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Other - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab129100 has not yet been referenced specifically in any publications.

      Publishing research using ab129100? Please let us know so that we can cite the reference in this datasheet.

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