RecombinantRabMAb

Recombinant Anti-ZNF136 antibody [EPR8225] (ab129100)

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Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
  • OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
  • Anti-ZNF136 antibody [EPR8225] (ab129100)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8225] to ZNF136
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ZNF136 antibody [EPR8225]
    See all ZNF136 primary antibodies

  • Description

    Rabbit monoclonal [EPR8225] to ZNF136

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ZNF136 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal liver and 293T cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.

    • Tissue specificity

      Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 14 C2H2-type zinc fingers.
      Contains 1 KRAB domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • pHZ 20 antibody
        • Zinc finger protein 136 antibody
        • Zinc finger protein 136 clone pHZ 20 antibody
        • ZN136_HUMAN antibody
        • ZNF136 antibody
        see all

      Images

      • Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Western blot - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Lanes 1-3 : Anti-ZNF136 antibody [EPR8225] (ab129100) at 1/1000 dilution
        Lane 4 : Anti-ZNF136 antibody [EPR8225] (ab129100)

        Lane 1 : Human fetal heart cell lysates at 10 µg
        Lane 2 : Human fetal liver cell lysates at 10 µg
        Lane 3 : 293T cell lysates at 10 µg
        Lane 4 : HeLa cell lysates

        Secondary
        Lanes 1-3 : Goat anti-Rabbit HRP at 1/2000 dilution


        Predicted band size: 63 kDa

      • OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
        OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] (ab129100)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ZNF136 antibody [EPR8225] (ab129100)
        Anti-ZNF136 antibody [EPR8225] (ab129100)

      References (0)

      Publishing research using ab129100? Please let us know so that we can cite the reference in this datasheet.

      ab129100 has not yet been referenced specifically in any publications.

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