Overview

  • Product name

  • Description

    Rabbit polyclonal to ZNF148
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human ZNF148 aa 20-131.
    Sequence:

    MQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEM RQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEIT FTDVSEQLMRDK


    Database link: Q9UQR1

  • Positive control

    • ICC/IF: U-2 OS cells. IHC-P: Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab223706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/permeabilization: PFA/Triton X-100

Target

Images

  • Paraffin embedded sections of human kidney were stained for ZNF148 with ab223706 at 1/20 dilution in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ZNF148 (green) using ab223706 at 4 µg/ml in ICC/IF.

References

ab223706 has not yet been referenced specifically in any publications.

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