Anti-ZNF148 antibody (ab227076)
Key features and details
- Rabbit polyclonal to ZNF148
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF148 antibody
See all ZNF148 primary antibodies -
Description
Rabbit polyclonal to ZNF148 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human ZNF148 (N terminal). The exact sequence is proprietary.
Database link: Q9UQR1 -
Positive control
- WB: HEK-293T whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 613265 Cow
- Entrez Gene: 7707 Human
- Omim: 601897 Human
- SwissProt: Q3Y4E1 Cow
- SwissProt: Q9UQR1 Human
- Unigene: 592591 Human
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Alternative names
- Transcription factor ZBP-89 antibody
- Transcription factor ZBP89 antibody
- ZBP89 antibody
see all
Images
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Anti-ZNF148 antibody (ab227076) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 89 kDa -
Paraffin-embedded human breast cancer tissue stained for ZNF148 with ab227076 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNF148 (green) using ab227076 at1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227076 has not yet been referenced specifically in any publications.