Overview

  • Product name

  • Description

    Rabbit polyclonal to ZNF148
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control

    • Human brain tissue HepG2 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab69933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

Images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab69933 at 1/50 dilution. Right panel was treated with immunising peptide.
  • All lanes : Anti-ZNF148 antibody (ab69933) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Salmon M  et al. Klf4, Klf2, and Zfp148 activate autophagy-related genes in smooth muscle cells during aortic aneurysm formation. Physiol Rep 7:e14058 (2019). Read more (PubMed: 31025534) »
  • Gao XH  et al. ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer. Medicine (Baltimore) 96:e5845 (2017). Read more (PubMed: 28072746) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 18 2012

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